Structural and biochemical characterization of mitochondrial citrate synthase 4 from Arabidopsis thaliana.

Acta Crystallographica. Section F, Structural Biology Communications
Kazuya Nishio, Tsunehiro Mizushima

Abstract

Citrate synthase (CS) catalyzes the conversion of oxaloacetate and acetyl coenzyme A into citrate and coenzyme A in the mitochondrial tricarboxylic acid (TCA) cycle. In plants, mitochondrial metabolism, including the TCA cycle, occurs in interaction with photosynthetic metabolism. The controlled regulation of several enzymes in the TCA cycle, such as CS, is important in plants. Here, the first crystal structure of a plant mitochondrial CS, CSY4 from Arabidopsis thaliana (AtCSY4), has been determined. Structural comparison of AtCSY4 with mitochondrial CSs revealed a high level of similarity. Inhibition analysis showed a similar manner of inhibition as in mitochondrial CSs. The effect of oxidation on one of a pair of cysteine residues in AtCSY4 was speculated upon based on the folded structure.

References

Apr 1, 1991·Archives of Biochemistry and Biophysics·G Kispal, P A Srere
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·G Wiegand, S J Remington
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yves BalmerBob B Buchanan
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Jun 14, 2006·Biochemistry·Bryan SchmidtPhilip J Hogg
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
May 2, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Steven B LarsonAlexander McPherson
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Mar 18, 2011·Plant, Cell & Environment·Renate Scheibe, Karl-Josef Dietz
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Dec 14, 2012·Antioxidants & Redox Signaling·Markus Schwarzländer, Iris Finkemeier
Nov 8, 2013·Molecular Plant·Elisabeth SchmidtmannIris Finkemeier
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
May 2, 2014·Nucleic Acids Research·Marco BiasiniTorsten Schwede
Nov 6, 2018·Nucleic Acids Research·UNKNOWN UniProt Consortium
Oct 8, 2019·Acta Crystallographica. Section D, Structural Biology·Dorothee LiebschnerPaul D Adams

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.