Structural and biological characterization of two novel peptides from the venom of the neotropical social wasp Agelaia pallipes pallipes

Toxicon : Official Journal of the International Society on Toxinology
Maria Anita MendesMario Sergio Palma

Abstract

The venom of the neotropical social wasp Agelaia pallipes pallipes was fractionated by RP-HPLC resulting in the elution of seven fractions; the last two were re-fractionated under RP-HPLC by using isocratic elution conditions and the purity of the fractions were confirmed by using ESI-MS analysis. Both fractions are constituted of peptide components, which were sequenced by Edman degradation chemistry, resulting in the following sequences: Protonectin I-L-G-T-I-L-G-L-L-K-G-L-NH(2). Agelaia-MP I-N-W-L-K-L-G-K-A-I-I-D-A-L-NH(2). Both peptides are manually synthesized on solid-phase and functionally characterized by using Wistar rats cells. Protonectin is a non-hemolytic chemotactic peptide for polymorphonucleated leukocytes (PMNL), presenting some mast cell degranulating activity and potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Agelaia-MP was characterized as a hemolytic mast cell degranulator toxin, presenting a poor antimicrobial action and no chemotaxis for PMNL.

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Citations

May 2, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Deepak Kumar KaushikAnirban Basu
Jun 23, 2011·Journal of Proteomics·Nicoli Barão Baptista-SaidembergMario Sergio Palma
Feb 13, 2009·Journal of the American Chemical Society·Rachel S Signarvic, William F Degrado
Apr 5, 2005·Applied Microbiology and Biotechnology·Ingrid SvenssonLena Häggström
May 26, 2010·Amino Acids·Petr NiederhafnerJiřina Slaninová
Jul 6, 2010·Toxicon : Official Journal of the International Society on Toxinology·Nicoli B Baptista-SaidembergMario S Palma
Oct 28, 2009·Toxicon : Official Journal of the International Society on Toxinology·Ji Hyeong Baek, Si Hyeock Lee
Mar 5, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xinbo YangHaining Yu
Dec 27, 2008·Toxicon : Official Journal of the International Society on Toxinology·Reto StöcklinDietrich Mebs
Aug 30, 2008·Peptides·Wenhu ChenHaining Yu
Jun 19, 2007·Toxicon : Official Journal of the International Society on Toxinology·Thalita RochaMaria Alice da Cruz-Höfling
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