Structural and biomechanical characterizations of porcine myocardial extracellular matrix.

Journal of Materials Science. Materials in Medicine
Bo WangJun Liao

Abstract

Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson's trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat's pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine α-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.

References

Jun 1, 1989·Journal of the American College of Cardiology·K T Weber
Mar 1, 1969·Circulation Research·D D StreeterE H Sonnenblick
Aug 1, 1995·Journal of Biomedical Materials Research·S F BadylakC Simmons
Feb 1, 1995·The Annals of Thoracic Surgery·G J CooperP H Deleuze
May 14, 1993·Science·R Langer, J P Vacanti
Mar 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·J R GlassonD C Miller
May 9, 1998·Journal of Molecular and Cellular Cardiology·A G VouyoukaB E Sumpio
Jul 15, 1999·Biotechnology and Bioengineering·R L CarrierG Vunjak-Novakovic
Apr 25, 2000·Cardiovascular Research·Y Sun, K T Weber
May 4, 2001·Journal of Biomaterials Science. Polymer Edition·D W Hutmacher
Sep 18, 2001·Heart·G Macchiarelli, O Ohtani
Apr 12, 2002·Journal of Biomedical Materials Research·Wan-Ju LiFrank K Ko
May 11, 2002·Journal of Biomechanical Engineering·Blayne A RoederSherry L Voytik-Harbin
Nov 26, 2002·The Journal of Thoracic and Cardiovascular Surgery·Tsukasa OzawaRen-Ke Li
Feb 26, 2003·Circulation·Bodo E Strauer, Ran Kornowski
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Sibylle GradMauro Alini
Dec 31, 2003·Biomaterials·Wolfram Hubertus ZimmermannThomas Eschenhagen
Feb 6, 2004·Plastic and Reconstructive Surgery·Gregory H BorschelWilliam M Kuzon
May 26, 2004·Transplant Immunology·Stephen F Badylak
Oct 7, 2004·The Annals of Thoracic Surgery·Laurent BarandonCécile Duplàa
Jul 7, 2005·Tissue Engineering·Gregory H BorschelDavid L Brown
Jul 12, 2005·Annual Review of Biomedical Engineering·Jeffrey W HolmesJames W Covell
Nov 4, 2005·Artificial Organs·Ravi K BirlaRobert G Dennis
Mar 8, 2006·Biomaterials·Thomas W GilbertStephen F Badylak
Apr 25, 2006·Lancet·Anthony AtalaAlan B Retik
Jun 15, 2006·Plastic and Reconstructive Surgery·Gregory H BorschelDavid L Brown
Dec 30, 2006·Circulation·Wayne RosamondUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
Jan 30, 2007·The Annals of Thoracic Surgery·Kazuro L FujimotoWilliam R Wagner
Mar 9, 2007·World Journal of Surgery·Xinwen WangChangyi Chen
Jul 24, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Robert W GraussDouwe E Atsma
Sep 6, 2007·Stem Cells and Development·Rishi Sharma, Ram Raghubir

❮ Previous
Next ❯

Citations

Mar 8, 2013·Heart Failure Reviews·Ernest Adeghate, Jaipaul Singh
Dec 4, 2014·BioResearch Open Access·Ketaki MetheSuchitra Sumitran-Holgersson
Mar 5, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Avione Y LeeChristopher K Breuer
Jun 10, 2015·Advanced Drug Delivery Reviews·Raymond M Wang, Karen L Christman
Dec 15, 2015·Advanced Drug Delivery Reviews·A K CapulliK K Parker
Nov 19, 2015·Journal of Molecular and Cellular Cardiology·Margaux A Horn, Andrew W Trafford
Nov 5, 2013·Biomaterials·Sareh Rajabi-ZeletiNasser Aghdami
Mar 6, 2015·Critical Reviews in Biotechnology·Sonia ZiaAlexander M Seifalian
May 10, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sen WangXiong Guo
May 18, 2017·Biomaterials Science·Gigi Chi Ting Au-YeungSubbu S Venkatraman
Apr 25, 2018·Stem Cells Translational Medicine·Clotilde Castaldo, Isotta Chimenti
Aug 24, 2018·Neural Regeneration Research·Jongbae ChoiSung Won Kwon
Jul 26, 2018·Artificial Organs·Doris A TaylorLuiz C Sampaio
Feb 5, 2019·Advanced Healthcare Materials·Donald Bejleri, Michael E Davis
May 8, 2018·Advanced Healthcare Materials·Jesus Paez-MayorgaAli Khademhosseini
Oct 4, 2012·Expert Review of Cardiovascular Therapy·Mai T Lam, Joseph C Wu
Nov 6, 2018·Scientific Reports·Mickey ShahGe Zhang
Nov 24, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ting LiQiang Ao
Mar 7, 2017·Current Stem Cell Reports·Doris A TaylorLuiz C Sampaio
Jan 31, 2021·Journal of the Mechanical Behavior of Biomedical Materials·Bryn L BrazileJun Liao
Jan 21, 2021·International Journal of Molecular Sciences·Georgia PennarossaTiziana A L Brevini
Feb 12, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Priya Mohindra, Tejal A Desai
Feb 6, 2018·Acta Biomaterialia·Jhilmil Dhulekar, Agneta Simionescu
Jun 15, 2021·Frontiers in Immunology·Maria Luisa BarcenaVera Regitz-Zagrosek
Aug 9, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Bo WangJun Liao
Dec 29, 2016·ACS Applied Materials & Interfaces·Pawan KcGe Zhang
Dec 15, 2020·ACS Biomaterials Science & Engineering·Cancan XuYi Hong
Sep 4, 2021·Tissue Engineering. Part B, Reviews·Tori SalemJared M Churko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.