Structural and biophysical insights into the role of the insert region in Rac1 function

Biochemistry
Roopa ThaparSharon L Campbell

Abstract

A 13 amino acid insertion that forms a short 3(10) helix between beta-strand 5 and alpha-helix 4 is a distinguishing feature among most members of the Rho family of GTPases, yet the precise role of this region in signal transduction is poorly understood. Previous in vivo functional studies have implicated the insert region of RhoA, Rac1, and Cdc42 to be important for cell transformation, regulation of the actin cytoskeleton, controlling DNA synthesis, and in the activation of downstream targets. In Rac1, our recent biological studies have suggested that the insert is important for SRF activation and the formation of lamellipodia but is dispensable for all other cellular functions of this protein. In the studies reported herein, we have characterized the effect of the insert deletion on Rac1 structure, thermodynamic stability, and the kinetics of nucleotide association. These in vitro studies help clarify biological data and provide further insights as to the role of this 13 amino acid region in modulating Rac1 function. The studies reveal that deletion of the insert has no effect on Rac1 structure and causes only a marginal (approximately 0.8 kcal/mol) decrease in the DeltaG(fold) of Rac1*GDP*Mg2+. The intrinsic rate of nucleot...Continue Reading

References

Aug 1, 1996·Journal of Biochemistry·S Narumiya
May 23, 1998·The Journal of Biological Chemistry·K IharaT Hakoshima
Nov 7, 1998·The Journal of Biological Chemistry·K MissyB Payrastre
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·J H Hurley, S Misra

❮ Previous
Next ❯

Citations

Nov 8, 2002·Genome Research·Ilya G SerebriiskiiErica A Golemis
Jan 25, 2005·Journal of Neurochemistry·Jeffrey McKinneyJan Haavik
Feb 15, 2008·Plant Physiology and Biochemistry : PPB·Antje Berken, Alfred Wittinghofer
Aug 16, 2016·Structure·Louis LeCourDavid K Worthylake
Oct 12, 2018·Biochemical Society Transactions·Helen R Mott, Darerca Owen
Oct 13, 2007·The Journal of Biological Chemistry·Adam ShutesChanning J Der
Jul 25, 2006·The Journal of Biological Chemistry·Carla V FinkielsteinDaniel G S Capelluto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.