Structural and cell-associated proteins of Lassa virus

The Journal of General Virology
J C Clegg, G Lloyd

Abstract

Lassa virus was purified from culture fluids of infected CV-1 monkey kidney cells and its structural proteins analysed by polyacrylamide gel electrophoresis. Stained gels showed a typical arenavirus profile, with a prominent protein of molecular weight 60000, corresponding to the nucleocapsid protein N, and two faint broad bands with molecular weights of 45000 and 38000, the envelope glycoproteins G1 and G2. G1 and G2 were shown to be glycosylated by their ability to bind concanavalin A to nitrocellulose transfers of the separated proteins ('Western blots'). N and G2 bound antibody from guinea-pig or human convalescent sera but G1 was inactive, presumably as a result of denaturation. This technique also revealed other apparently virus-specific minor bands with molecular weights of 76000 and 68000. When Western blots of proteins of infected cells which had been lysed in SDS were probed with anti-Lassa virus serum or stained for glycoproteins, four virus-specific bands were apparent: the N, G1 and G2 proteins seen in purified virus, and a glycoprotein of molecular weight 72000 which probably corresponds to the envelope protein precursor (GPC) seen in other arenavirus systems. Immunoprecipitates from infected CV-1 cells labelled w...Continue Reading

Citations

Jan 1, 1985·Archives of Virology·I S Lukashevich, N N Lemeshko
Jan 1, 1986·Medical Microbiology and Immunology·I S LukashevichE P Stcheslyenok
Jan 1, 1986·Medical Microbiology and Immunology·J P GonzalezJ B McCormick
Jan 1, 1984·Transactions of the Royal Society of Tropical Medicine and Hygiene·J C Clegg
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Dec 9, 2014·Human Vaccines & Immunotherapeutics·Pierre LeblancMark C Poznansky
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Jul 13, 2018·PLoS Pathogens·María Eugenia LoureiroElina Zúñiga
Jan 18, 2019·Pathogens·Dylan M JohnsonIgor S Lukashevich
Oct 31, 2017·Microbes and Infection·Bjoern Meyer, Allison Groseth

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