PMID: 6404305Apr 28, 1983Paper

Structural and chromatographic heterogeneity of normal plasma fibrinogen associated with the presence of three gamma-chain types with distinct molecular weights

Biochimica Et Biophysica Acta
Charles W FrancisV J Marder

Abstract

Three different gamma chains have been identified in fibrinogen isolated from normal human plasma with apparent molecular weights of 50000 (gamma 50), 55000 (gamma 55) and 57500 (gamma 57.5), as shown by SDS-polyacrylamide gel electrophoresis. Plasma fibrinogen was separated by ion-exchange chromatography on DEAE-Sephacel into three populations of molecules, each differing in gamma-chain composition. The first peak contained 87% of the total fibrinogen and was composed of molecules containing only gamma 50 chains; the second peak included 3% of the fibrinogen and contained one gamma 50 and one gamma 55 chain, and the third peak had 10% of the total which contained one gamma 50 and one gamma 57.5 chain. Cross-linked fibrin obtained from fibrinogen with only gamma 50 chains contained gamma gamma dimers exclusively of Mr 100000, representing a uniform gamma 50 gamma 50 dimer composition. The gamma gamma dimers from purified fibrinogen of gamma 50 gamma 55 type had molecular weights of 111000, 105000 and 100000, while dimers from gamma 50 gamma 57.5 fibrinogen were of Mr 115000, 108000 and 100000. The relative proportions of gamma gamma dimers from each purified fibrinogen population were consistent with random crosslinking of the ...Continue Reading

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Citations

Jul 4, 2001·Thrombosis Research·D A MehG Di Minno
Jan 1, 1986·Critical Reviews in Oncology/hematology·A Z Budzynski
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B H HorwitzH A Scheraga
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C W FrancisV J Marder
Aug 19, 2009·Blood·Shirley Uitte de WilligeRobert A S Ariëns
Oct 14, 2006·Journal of Thrombosis and Haemostasis : JTH·M W MosessonR M Bertina
Jan 1, 1988·Critical Reviews in Clinical Laboratory Sciences·J A Shafer, D L Higgins
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·M W Mosesson

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