Jun 9, 2020Paper

Structural and functional analysis of a potent sarbecovirus neutralizing antibody

BioRxiv : the Preprint Server for Biology
Dora PintoDavide Corti

Abstract

SARS-CoV-2 is a newly emerged coronavirus responsible for the current COVID-19 pandemic that has resulted in more than one million infections and 73,000 deaths 1,2 . Vaccine and therapeutic discovery efforts are paramount to curb the pandemic spread of this zoonotic virus. The SARS-CoV-2 spike (S) glycoprotein promotes entry into host cells and is the main target of neutralizing antibodies. Here we describe multiple monoclonal antibodies targeting SARS-CoV-2 S identified from memory B cells of a SARS survivor infected in 2003. One antibody, named S309, potently neutralizes SARS-CoV-2 and SARS-CoV pseudoviruses as well as authentic SARS-CoV-2 by engaging the S receptor-binding domain. Using cryo-electron microscopy and binding assays, we show that S309 recognizes a glycan-containing epitope that is conserved within the sarbecovirus subgenus, without competing with receptor attachment. Antibody cocktails including S309 along with other antibodies identified here further enhanced SARS-CoV-2 neutralization and may limit the emergence of neutralization-escape mutants. These results pave the way for using S309 and S309-containing antibody cocktails for prophylaxis in individuals at high risk of exposure or as a post-exposure therapy ...Continue Reading

Citations

Jun 7, 2020·Glycoconjugate Journal·Roberto Adamo, Sandro Sonnino
May 6, 2020·Science·Yasunori WatanabeMax Crispin
Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Sep 11, 2020·Science·Longxing CaoDavid Baker
Sep 11, 2020·Nature Genetics·Jason D FernandesMaximilian Haeussler
Sep 18, 2020·Oncoimmunology·Cléa MelenotteLaurence Zitvogel
Oct 27, 2020·Frontiers in Immunology·Nima TaefehshokrBryan Heit
Dec 17, 2020·Frontiers in Cellular and Infection Microbiology·Mei-Yue WangJi-Min Cao
Jan 5, 2021·Frontiers in Molecular Biosciences·Giuseppina MarianoJulien R C Bergeron
Feb 18, 2021·Reviews in Medical Virology·Arash ArashkiaSARS CoV-2 Rapid Response Team of Pasteur Institute of Iran (PII)
May 28, 2021·Frontiers in Molecular Biosciences·Perumal GobinathRadhakrishnan Surendrakumar

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