PMID: 3755819Aug 11, 1986Paper

Structural and functional analysis of an alternatively spliced chicken TK messenger RNA

Nucleic Acids Research
G F Merrill, F D Tufaro

Abstract

The nucleotide sequence of independent cDNA clones revealed that TK transcripts can undergo alternative splicing within the 3' nontranslated portion of the message. The most abundant mRNA is 2.1 kb in length and is colinear with the underlying genomic sequence for at least the first 1052 bases of its 3' nontranslated region. A less abundant mRNA, 1.3 kb in length, differs from the larger mRNA in that an 863 base intron is spliced from the 3' nontranslated region. The possibility that removal of the alternatively spliced intron allows the small mRNA to persist in postreplicative cells was investigated in two ways. First, the levels of large and small TK mRNA in tissues expressing differential growth rates was determined. Second, the pattern of TK enzyme expression during differentiation was analyzed in muscle cells transformed with recombinant TK genes lacking the 3' nontranslated intron. Both lines of experimentation indicated that the small TK mRNA was as dependent as the large TK mRNA on the replicative state of the cell.

References

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Citations

May 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G F Merrill
Apr 1, 1990·Archives of Biochemistry and Biophysics·K Ito, K Igarashi
Jun 1, 1989·Japanese Journal of Cancer Research : Gann·G B KnightA B Pardee
Jan 1, 1987·Annals of the New York Academy of Sciences·D Rosson, E P Reddy

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