Structural and functional changes in RNAse A originating from tyrosine and histidine cross-linking and oxidation induced by singlet oxygen and peroxyl radicals

Free Radical Biology & Medicine
Fabian LeinischMichael J Davies

Abstract

Oxidation can be induced by multiple processes in biological samples, with proteins being important targets due to their high abundance and reactivity. Oxidant reactions with proteins are not comprehensively understood, but it is known that structural and functional changes may be a cause, or a consequence, of disease. The mechanisms of oxidation of the model protein RNAse A by singlet oxygen (1O2) were examined and compared to peroxyl radical (ROO•) oxidation, both common biological oxidants. This protein is a prototypic member of the RNAse family that exhibits antiviral activity by cleaving single-stranded RNA. RNAse A lacks tryptophan and cysteine residues which are major oxidant targets, but contains multiple histidine, tyrosine and methionine residues; these were therefore hypothesized to be the major sites of damage. 1O2 and ROO• induce different patterns and extents of damage; both induce cross-links and side-chain oxidation, and 1O2 exposure modulates enzymatic activity. Multiple products have been characterized including methionine sulfoxide and sulfone, alcohols, DOPA, 2-oxohistidine, histidine-derived ring-opened species and inter- and intra-molecular cross-links (di-tyrosine, histidine-lysine, histidine-arginine, ty...Continue Reading

Citations

Aug 10, 2018·Expert Review of Proteomics·Per HägglundMichael J Davies
Dec 18, 2019·Essays in Biochemistry·Eduardo Fuentes-Lemus, Camilo López-Alarcón
Feb 6, 2020·Pharmaceutical Research·Christian Schöneich
Nov 2, 2019·The Journal of Biological Chemistry·Clare L Hawkins, Michael J Davies
Aug 26, 2019·Free Radical Biology & Medicine·Eduardo Fuentes-LemusMichael J Davies
Aug 11, 2020·Free Radical Biology & Medicine·Camilo López-AlarcónMichael J Davies
Sep 3, 2020·Free Radical Biology & Medicine·Luke CarrollMichael J Davies
Feb 19, 2021·Redox Biology·Shuwen JiangMichael J Davies
Nov 5, 2020·Environmental Science & Technology·Christine M EgliElisabeth M L Janssen
Aug 3, 2019·Environmental Science & Technology·Mercedes RuizJoel A Pedersen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.