Structural and functional characterisation of the voltage sensor in the ion channel human annexin V

Journal of Molecular Biology
S LiemannP Göttig

Abstract

The ion channel properties of human annexin V, a calcium- and phospholipid-binding protein of the annexin family, have been structurally and functionally investigated by analysing the mutant Glu112 -->Gly. Glu112 forms a salt bridge with Arg271 located in the interior of the hydrophilic pore of the molecule which is conserved within the annexin family. The crystal structures of the mutant and wild-type proteins are very similar and show only marginal conformational changes around the mutation site. Electron microscopic images show a conserved four-domain structure upon membrane binding as in the wild-type annexin V. The channel properties of the mutant are drastically changed, as the mutant has lost the voltage-dependent channel gating and the selectivity for calcium ions over monovalent cations. These results strongly support the hypothesis that the central, hydrophilic pore is the ion-conducting pathway.

Citations

Dec 17, 1998·The Biochemical Journal·M I Rodriguez-GarciaM P Fernandez
Feb 24, 2009·The Plant Cell·Anuphon LaohavisitJulia M Davies
Apr 1, 2003·Molecular and Cellular Biology·Bent BrachvogelErnst Pöschl
Jan 22, 2009·Journal of Neuroinflammation·Lawrence L HorstmanAlireza Minagar
Jan 30, 2013·International Journal of Molecular Sciences·María Antonia LizarbeJavier Turnay
Feb 13, 2008·Journal of Experimental Botany·Jennifer C MortimerJulia M Davies
Nov 10, 2005·Autoimmunity·Borut BozicBlaz Rozman
Nov 10, 2006·Biochimica Et Biophysica Acta·Reginald O MorganMaria-Pilar Fernandez
Sep 27, 2002·Biophysical Journal·Javier TurnayM Antonia Lizarbe
Jun 24, 2004·Journal of Cellular Biochemistry·Jennifer M GilletteSheila M Nielsen-Preiss
Sep 22, 1998·FEBS Letters·R O Morgan, M P Fernandez
Nov 14, 2007·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Behcet SimsekAli Anarat
Mar 29, 2001·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·R MatsudaN Ogawa
Nov 19, 2010·The New Phytologist·Anuphon Laohavisit, Julia M Davies
Aug 26, 2000·Progress in Biophysics and Molecular Biology·J I Kourie, H B Wood
Oct 10, 2009·Plant Signaling & Behavior·Jennifer C MortimerJulia M Davies
Dec 22, 1999·Biochemical and Biophysical Research Communications·C Gidon-JeangirardM C Martínez
Aug 28, 1997·Biochemical and Biophysical Research Communications·R MatsudaY Horikawa
May 18, 1999·Journal of Molecular Biology·A RosengarthV Gerke
Jun 9, 2001·Biochemical and Biophysical Research Communications·M GolczakS Pikula
Jul 6, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·R MatsudaT Ieiri

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