Structural and Functional Characterization of the BcsG Subunit of the Cellulose Synthase in Salmonella typhimurium

Journal of Molecular Biology
Lei SunUte Römling

Abstract

Many bacteria secrete cellulose, which forms the structural basis for bacterial multicellular aggregates, termed biofilms. The cellulose synthase complex of Salmonella typhimurium consists of the catalytic subunits BcsA and BcsB and several auxiliary subunits that are encoded by two divergently transcribed operons, bcsRQABZC and bcsEFG. Expression of the bcsEFG operon is required for full-scale cellulose production, but the functions of its products are not fully understood. This work aimed to characterize the BcsG subunit of the cellulose synthase, which consists of an N-terminal transmembrane fragment and a C-terminal domain in the periplasm. Deletion of the bcsG gene substantially decreased the total amount of BcsA and cellulose production. BcsA levels were partially restored by the expression of the transmembrane segment, whereas restoration of cellulose production required the presence of the C-terminal periplasmic domain and its characteristic metal-binding residues. The high-resolution crystal structure of the periplasmic domain characterized BcsG as a member of the alkaline phosphatase/sulfatase superfamily of metalloenzymes, containing a conserved Zn2+-binding site. Sequence and structural comparisons showed that BcsG ...Continue Reading

Citations

Jun 25, 2019·PLoS Genetics·Keith D MacKenzieAaron P White
Mar 30, 2020·Applied Microbiology and Biotechnology·Stefanie B Costa-GutierrezRicardo E de Cristóbal
Mar 11, 2020·The Journal of Biological Chemistry·Alexander C AndersonJoel T Weadge
Jan 12, 2021·Current Opinion in Structural Biology·Sean D Liston, Lisa M Willis
Mar 14, 2021·Nature Structural & Molecular Biology·Justin F AchesonJochen Zimmer
Aug 4, 2021·Annual Review of Microbiology·Diego O Serra, Regine Hengge
Oct 12, 2021·FEMS Microbiology Reviews·Wiem AbidiPetya Violinova Krasteva

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