PMID: 7541912Jun 25, 1995Paper

Structural and functional characterization of the promoter regions of the NFKB2 gene

Nucleic Acids Research
L LombardiA Neri

Abstract

In order to clarify the transcriptional regulation of the NFKB2 gene (lyt-10, NF-kappa Bp100), we have characterized the structure and function of its promoter regions. Based on the nucleotide sequence of cDNA clones and the 5' flanking genomic region of the NFKB2 gene, RT-PCR analysis in a number of human cell lines demonstrated the presence of two alternative noncoding first exons (1a and 1b). Two distinct promoter regions, P1 and P2, were identified upstream of each exon, containing multiple sites of transcription initiation, as shown by RNase protection analysis. Sequence analysis of these regions showed a CAAT box upstream of exon 1a and high G-C content regions within both P1 and P2. Consensus binding sites for transcription factors, including SP1, AP1 and putative NF-kappa B (kappa B sites), were found upstream of each exon. In particular, six kappa B sites were identified, all but one of them capable of binding NF-kappa B complexes in vitro. Transfection in HeLa cells of plasmids containing P1 and P2 sequences linked to a chloramphenicol acetyltransferase reporter gene indicated that both P1 and P2 can act independently as promoters. Co-transfection of NF-kappa B effector plasmids (NF-kappa Bp52 and RelA) with a reporte...Continue Reading

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Citations

Jun 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Vincent F-S ShihAlexander Hoffmann
Apr 1, 2010·The Journal of Biological Chemistry·Sarah Jill de JongBrigitte Biesinger
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Mar 23, 2012·Immunological Reviews·Mary Kaileh, Ranjan Sen
Mar 23, 2012·Immunological Reviews·Michael HinzClaus Scheidereit
Apr 22, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carine PoussinMichael Lietz
Oct 22, 2013·Molecular and Cellular Endocrinology·Lacey M LitchfieldCarolyn M Klinge
Oct 22, 2016·In Vitro Cellular & Developmental Biology. Animal·Josiane Lilian Dos Santos SchiavinatoRodrigo Alexandre Panepucci
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