Structural and functional characterization of two-domain laccase from Streptomyces viridochromogenes

Biochimie
L I TrubitsinaA Leontievsky

Abstract

Laccase (EC 1.10.3.2) is one of the most common copper-containing oxidases found in many organisms and catalyses oxidation of primarily phenolic compounds by oxygen. A recently found bacterial laccase whose molecule is formed by two domains - the so called two-domain laccase (2DLac) or small laccase - has unusual resistance to inhibitors and an alkaline optimum of activity. The causes of these properties, as well as the biological function of two-domain laccases, are poorly understood. We performed an enzymatic and structural characterization of 2DLac from Streptomyces viridochromogenes (SvSL). It was cloned and overproduced in Escherichia coli. Phenolic compounds were oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds were oxidized at acidic but not alkaline pH. SvSL catalysed oxidation of nonphenolic compounds more efficiently than that of phenols. Moreover, this two-domain laccase displayed a cytochrome c oxidase activity and exhibited no ferroxidase activity. The enzyme was resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF. We succeeded in generating X-ray quality crystals and solved their structure to a resolution of 2.4 Å. S...Continue Reading

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Citations

Sep 2, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Svetlana TishchenkoAlexey Leontievsky
Mar 5, 2018·Applied Microbiology and Biotechnology·Jelena SpasicJasmina Nikodinovic-Runic
Nov 16, 2019·Microbial Cell Factories·Leticia ArreguiNorma A Valdez-Cruz
Aug 17, 2020·International Journal of Molecular Sciences·Verena BraunschmidGeorg M Guebitz
Feb 28, 2017·Israel Journal of Chemistry·Harry B Gray, Jay R Winkler
Feb 9, 2017·Scientific Reports·Rahul SinghLindsay D Eltis
Jul 3, 2019·International Journal of Molecular Sciences·Azat GabdulkhakovSvetlana Tishchenko
Apr 18, 2021·Biotechnology Letters·Asemahle GogotyaUchechukwu U Nwodo
Apr 20, 2021·Journal of Biomolecular Structure & Dynamics·Azat GabdulkhakovSvetlana Tishchenko
Oct 22, 2021·Frontiers in Microbiology·Chenxian YangXin Lü

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