Structural and functional consequences of EF-hand I recovery in mnemiopsin 2

International Journal of Biological Macromolecules
Vahab JafarianMahmoud Reza Aghamaali

Abstract

Mnemiopsin 2 from Mnemiopsis leidyi is a calcium-regulated photoprotein which has luminescence properties in the presence of calcium and coelenterazine. All calcium-regulated photoproteins contain EF-hand loops consisting of 12 individual residues in which the 6th position is occupied by Gly. However, the 6th residue in mneniopsin 2 is Glu rather than Gly. Here, we investigated the structural and functional consequences of substitution of Glu by Gly (E50G variant) using site-directed mutagenesis and spectroscopic procedures. It was revealed that the luminescence activity of the variant was about 17 times greater than that of wild-type (WT) photoprotein. In comparison with WT protein, our variant showed higher optimum temperature and calcium sensitivity as well as slower rate of luminescence decay. Homology modeling and sequence analysis with other known photoproteins showed that EF-hand I loop can affect the luminescence activity of E50G variant. Structural studies using circular dichroism and fluorescence spectroscopy revealed that mutation leads to the reduction in secondary structural content and local structural alterations. Finally, it can be concluded that the activity of E50G variant increases as a result of more flexibi...Continue Reading

References

Mar 1, 1990·Environmental Health Perspectives·J R Blinks
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 11, 1999·Nucleic Acids Research·D A BensonD L Wheeler
Dec 11, 1999·Nucleic Acids Research·A Bairoch, R Apweiler
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·M A Martí-RenomA Sali
Dec 15, 2000·Current Opinion in Structural Biology·A Lewit-Bentley, S Réty
Jun 1, 1962·Journal of Cellular and Comparative Physiology·O SHIMOMURAY SAIGA
Feb 4, 2005·Protein Science : a Publication of the Protein Society·Lu DengBi-Cheng Wang
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F I TsujiY Sakaki
May 9, 2006·Journal of Molecular Biology·Zenon Grabarek
Jun 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ludovic TricoireBertrand Lambolez
Feb 6, 2007·Protein Expression and Purification·Satoshi Inouye, Yuiko Sahara
Oct 14, 2008·Methods : a Companion to Methods in Enzymology·Marisa Brini
Aug 30, 2011·Biochemical and Biophysical Research Communications·Vahab JafarianSadegh Hassannia
Nov 28, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Atiyeh MahdaviReyhaneh Sariri
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Jan 31, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Atiyeh MahdaviMajid Taghdir
Dec 23, 2015·Journal of Photochemistry and Photobiology. B, Biology·Ludmila P BurakovaZhi-Jie Liu
May 18, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Masoomeh VafaVahab Jafarian

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Citations

Jun 7, 2019·Applied Microbiology and Biotechnology·Lyudmila P Burakova, Eugene S Vysotski

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