Structural and functional features of Rhodococcus ruber lipoarabinomannan

Microbiology
Kevin J C GibsonG S Besra

Abstract

The genus Rhodococcus is part of the phylogenetic group nocardioform actinomycetes, which also includes the genus MYCOBACTERIUM: Members of this phylogenetic group have a characteristic cell envelope structure, which is dominated by various complex lipids. Among these, lipoglycans are of particular interest since mycobacterial lipoarabinomannans are important immunomodulatory molecules that are likely to be involved in the subsequent fate of mycobacterial bacilli once inside phagocytic cells. Rhodococcus ruber is a species closely related to an established opportunistic human pathogen, Rhodococcus equi. This paper reports the isolation and characterization of R. ruber lipoarabinomannan, designated as RruLAM. SDS-PAGE and gas chromatography analyses revealed that RruLAM was of an intermediate size between Mycobacterium tuberculosis lipoarabinomannan and lipomannan. Using a combination of chemical degradation and (1)H, (13)C-NMR experiments, the carbohydrate structure of RruLAM was unambiguously shown to be composed of a linear (alpha1-->6)-Manp backbone substituted at some O-2 positions by a single t-alpha-Araf sugar unit. Integration of the anomeric proton signals provided an indication of the degree of branching as approximate...Continue Reading

References

Aug 1, 1978·The American Journal of Medicine·D R HaburchakE D Everett
Jun 1, 1989·The Journal of Applied Bacteriology·O U Osoagbaka
Aug 1, 1987·Veterinary Microbiology·M Goodfellow
Jan 1, 1995·Annual Review of Biochemistry·P J Brennan, H Nikaido
Sep 1, 1996·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·C FlahertyI C Sutcliffe
Aug 11, 1998·Frontiers in Bioscience : a Journal and Virtual Library·A VercelloneG Puzo
Dec 1, 1999·Glycoconjugate Journal·J NigouG Puzo
May 4, 2000·Systematic and Applied Microbiology·C Flaherty, I C Sutcliffe
Mar 10, 2001·Nature·S T ColeB G Barrell
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J NigouG Puzo
Feb 7, 2002·Journal of Molecular Biology·Achim TreumannSteve W Homans
Jun 20, 2002·The Journal of Biological Chemistry·Natalie J GartonIain C Sutcliffe

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Citations

Jan 22, 2005·Journal of Bacteriology·Martine GilleronIain C Sutcliffe
May 12, 2012·Organic & Biomolecular Chemistry·W Bruce Turnbull, Susanne A Stalford
Mar 20, 2012·Carbohydrate Research·Susanne PieperPetra Mischnick
Aug 31, 2010·Chemistry : a European Journal·Fabricio R BisognoVicente Gotor
Feb 24, 2015·Immunological Reviews·Ildiko Van Rhijn, D Branch Moody
May 24, 2005·Carbohydrate Research·Richard H FurneauxWayne B Severn
Jan 30, 2015·Frontiers in Cellular and Infection Microbiology·Isabelle VergneJérôme Nigou
Mar 20, 2004·The Journal of Biological Chemistry·Kevin J C GibsonJérôme Nigou
Jun 16, 2005·The Journal of Biological Chemistry·Kevin J C GibsonJérôme Nigou
Jun 27, 2019·Scientific Reports·Irina B IvshinaElena V Vikhareva
May 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jérôme NigouGermain Puzo
Feb 9, 2018·AIMS Microbiology·Lisa Ott

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