Structural and functional glycosphingolipidomics by glycoblotting with an aminooxy-functionalized gold nanoparticle

Biochemistry
Noriko NagahoriShin-Ichiro Nishimura

Abstract

Glycosphingolipids (GSLs) synthesized in Golgi apparatus by sequential transfer of sugar residues to a ceramide lipid anchor are ubiquitously distributing on vertebrate plasma membranes. A standardized method allowing for high-throughput structural profiling and functional characterization of living cell surface GSLs is of growing importance because they function as crucial signal transduction molecules in various processes of dynamic cellular recognitions. However, methods are not available for amplification of GSLs, while the genomic scale PCR amplification permits large-scale mammalian proteomic analysis. Here we communicate such an approach to a novel "omics", namely, glycosphingolipidomics based on the "glycoblotting" method. The method, which involves selective ozonolysis of the C-C double bond in the ceramide moiety and subsequent enrichment of generated GSL aldehydes by chemical ligation using an aminooxy-functionalized gold nanoparticle (aoGNP) should be of widespread utility for identifying and characterizing whole GSLs present in the living cell surfaces. The present protocol using glycoblotting permitted MALDI-TOFMS-based high-throughput structural profiling of mouse brain gangliosides such as GM1, GD1a/GD1b, and GT...Continue Reading

References

Apr 1, 1985·Analytical Biochemistry·S Ladisch, B Gillard
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S I Hakomori, W T Murakami
Feb 1, 1997·Chemistry & Biology·S Hakomori, Y Zhang
Sep 25, 1998·Science·M BruchezA P Alivisatos
Apr 20, 2000·The Journal of Biological Chemistry·D A Brown, E London
Jul 31, 2004·Analytical Chemistry·Chien-Hsun TengYu-Chie Chen
Nov 13, 2004·Angewandte Chemie·Eugenii Katz, Itamar Willner
Mar 18, 2005·Journal of the American Chemical Society·H Tetsuo UyedaHedi Mattoussi
Apr 14, 2005·Journal of the American Chemical Society·John A McLeanDavid H Russell
Apr 14, 2005·Chemistry : a European Journal·Kenichi NiikuraShin-Ichiro Nishimura
Jun 15, 2006·Chemistry : a European Journal·Noriko Nagahori, Shin-Ichiro Nishimura
Jan 17, 2007·Chemistry : a European Journal·Hideyuki ShimaokaShin-Ichiro Nishimura
Feb 15, 2007·Analytical Chemistry·Claire L SchofieldDavid A Russell
Mar 21, 2007·Chemistry : a European Journal·Yoshiaki MiuraShin-Ichiro Nishimura
May 25, 2007·Molecular & Cellular Proteomics : MCP·Yoko KitaShin-Ichiro Nishimura
Sep 22, 2007·Journal of Neurochemistry·Sathaporn NgamukoteRobert K Yu
Nov 8, 2007·Molecular & Cellular Proteomics : MCP·Yoshiaki MiuraShin-Ichiro Nishimura
Jun 18, 2001·Angewandte Chemie·Jesús M de la FuenteSoledad Penadés

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Citations

Jun 7, 2011·Analytical and Bioanalytical Chemistry·Rosa PilolliNicola Cioffi
Jul 12, 2011·Journal of the American Chemical Society·Tatsuya OhyanagiShin-ichiro Nishimura
Oct 20, 2009·The Analyst·Zheng-Jiang ZhuRichard W Vachet
Oct 21, 2009·Chemical Communications : Chem Comm·Mikkel B ThygesenKnud J Jensen
Apr 10, 2013·ACS Applied Materials & Interfaces·Kenichi NiikuraKuniharu Ijiro
Sep 13, 2011·Angewandte Chemie·Timothy M AltamoreTimothy Gallagher
Oct 9, 2012·Macromolecular Bioscience·Rajesh Sunasee, Ravin Narain
Dec 12, 2012·Chembiochem : a European Journal of Chemical Biology·Noriko NagahoriShin-Ichiro Nishimura
Apr 25, 2015·Biochimica Et Biophysica Acta·Solomon T GizawShin-Ichiro Nishimura
Nov 20, 2013·Biochimica Et Biophysica Acta·Takahiko MatsushitaShin-Ichiro Nishimura
Feb 2, 2017·Journal of Agricultural and Food Chemistry·Nagaraju BrijeshaHuligerepura Sosalegowda Aparna
Jan 1, 2012·Metabolites·Hany Farwanah, Thomas Kolter
Oct 11, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoshiko MiuraTomohiro Fukuda
Feb 22, 2013·Antiviral Chemistry & Chemotherapy·Fei FengShin-Ichiro Nishimura
Aug 6, 2017·Chembiochem : a European Journal of Chemical Biology·Takayuki FurukawaShu-Ping Hui
May 6, 2019·Electrophoresis·Siyao Liu, Michael Lämmerhofer
Feb 26, 2019·Drug Delivery·Xueqin ZhangHualiang Huang
Jan 11, 2013·Chemical Society Reviews·Niels C ReichardtSoledad Penadés
Jan 1, 2012·ISRN Biochemistry·Thomas Kolter
Jul 1, 2019·Particle & Particle Systems Characterization : Measurement and Description of Particle Properties and Behavior in Powders and Other Disperse Systems·Tirtha R SibakotiMichael H Nantz
Sep 28, 2020·Analytica Chimica Acta·Rodell C Barrientos, Qibin Zhang
Sep 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Aurélia PerinoAlain Wagner
Jul 7, 2018·Chemical Reviews·Carmanah D HunterChristopher W Cairo

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