Structural and functional insights into the mechanism of action of plant borate transporters.

Scientific Reports
S. SaourosBernadette Byrne

Abstract

Boron has essential roles in plant growth and development. BOR proteins are key in the active uptake and distribution of boron, and regulation of intracellular boron concentrations. However, their mechanism of action remains poorly studied. BOR proteins are homologues of the human SLC4 family of transporters, which includes well studied mammalian transporters such as the human Anion Exchanger 1 (hAE1). Here we generated Arabidopsis thaliana BOR1 (AtBOR1) variants based (i) on known disease causing mutations of hAE1 (S466R, A500R) and (ii) a loss of function mutation (D311A) identified in the yeast BOR protein, ScBOR1p. The AtBOR1 variants express in yeast and localise to the plasma membrane, although both S466R and A500R exhibit lower expression than the WT AtBOR1 and D311A. The D311A, S466R and A500R mutations result in a loss of borate efflux activity in a yeast bor1p knockout strain. A. thaliana plants containing these three individual mutations exhibit substantially decreased growth phenotypes in soil under conditions of low boron. These data confirm an important role for D311 in the function of the protein and show that mutations equivalent to disease-causing mutations in hAE1 have major effects in AtBOR1. We also obtained...Continue Reading

References

Nov 26, 2002·Nature·Junpei TakanoToru Fujiwara
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Junpei TakanoToru Fujiwara
Apr 27, 2007·American Journal of Physiology. Cell Physiology·Michael L JenningsRobyn Hannigan
Oct 30, 2007·Pflügers Archiv : European journal of physiology·Mayuki Tanaka, Toru Fujiwara
Dec 1, 2007·Science·Kyoko MiwaToru Fujiwara
Mar 17, 2010·Annals of Botany·Kyoko Miwa, Toru Fujiwara
Apr 30, 2015·Journal of Experimental Botany·Juan J Camacho-CristóbalAgustín González-Fontes
May 8, 2015·Methods in Enzymology·Aiman SadafPil Seok Chae
Aug 19, 2015·Journal of Structural Biology·Alexis Rohou, Nikolaus Grigorieff
Sep 15, 2015·Nature Structural & Molecular Biology·Eric R GeertsmaRaimund Dutzler
Mar 30, 2016·Annual Review of Biochemistry·David Drew, Olga Boudker
Sep 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Bryan H Thurtle-Schmidt, Robert M Stroud
Oct 22, 2016·Protein Science : a Publication of the Protein Society·Nicolas CoudrayDavid L Stokes
Mar 4, 2018·Nature Communications·Kevin W HuynhIra Kurtz
Apr 14, 2018·Molecular Membrane Biology·Xiaoyun BaiReinhart A F Reithmeier
Jan 4, 2019·Nature Communications·Maksim KunitskiReinhard Dörner
Oct 28, 2019·Protein Expression and Purification·Savvas SaourosBernadette Byrne
Nov 13, 2019·Nature Plants·Chengcheng WangPeng Zhang
May 15, 2020·Current Opinion in Cell Biology·Ben P PhillipsElizabeth A Miller
May 2, 2021·FEBS Open Bio·Cristina CecchettiBernadette Byrne

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