Structural and functional insights into Saccharomyces cerevisiae Tpa1, a putative prolylhydroxylase influencing translation termination and transcription.

The Journal of Biological Chemistry
Julien HenriMarc Graille

Abstract

Efficiency of translation termination relies on the specific recognition of the three stop codons by the eukaryotic translation termination factor eRF1. To date only a few proteins are known to be involved in translation termination in eukaryotes. Saccharomyces cerevisiae Tpa1, a largely conserved but uncharacterized protein, has been described to associate with a messenger ribonucleoprotein complex located at the 3' end of mRNAs that contains at least eRF1, eRF3, and Pab1. Deletion of the TPA1 gene results in a decrease of translation termination efficacy and an increase in mRNAs half-lives and longer mRNA poly(A) tails. In parallel, Schizosaccharomyces pombe Ofd1, a Tpa1 ortholog, and its partner Nro1 have been implicated in the regulation of the stability of a transcription factor that regulates genes essential for the cell response to hypoxia. To gain insight into Tpa1/Ofd1 function, we have solved the crystal structure of S. cerevisiae Tpa1 protein. This protein is composed of two equivalent domains with the double-stranded β-helix fold. The N-terminal domain displays a highly conserved active site with strong similarities with prolyl-4-hydroxylases. Further functional studies show that the integrity of Tpa1 active site as...Continue Reading

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jan 27, 1989·Cell·K PfeiferL Guarente
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·S S EaglestoneM F Tuite
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·J DíezP Ahlquist
Jun 22, 2000·Yeast·V V Kushnirov
Nov 21, 2000·Trends in Biochemical Sciences·L L Kisselev, R H Buckingham
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·L Zhang, A Hach
Jun 14, 2001·Methods : a Companion to Methods in Enzymology·O PuigB Séraphin
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider, George M Sheldrick
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Roberto A SteinerBauke W Dijkstra
Jan 7, 2003·The EMBO Journal·Lev KisselevLudmila Frolova
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Johanna Myllyharju
Oct 24, 2003·The Journal of Biological Chemistry·Hai PangZihe Rao
Oct 24, 2003·Acta Crystallographica. Section D, Biological Crystallography·G BricogneW Paciorek
Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·David B KushnerPaul Ahlquist
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Gladys I. Cassab
May 4, 2004·Critical Reviews in Biochemistry and Molecular Biology·Robert P Hausinger
May 4, 2004·Nature Reviews. Molecular Cell Biology·Christopher J Schofield, Peter J Ratcliffe
Nov 27, 2004·The EMBO Journal·Andrzej DziembowskiBertrand Séraphin
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jun 15, 2005·Annual Review of Biochemistry·William G Kaelin
Dec 21, 2005·Molecular Cell·Marc GrailleRichard H Buckingham
Mar 4, 2006·Journal of Inorganic Biochemistry·Ian J CliftonChristopher J Schofield
Mar 16, 2006·Molecular and Cellular Biology·Bridget L ToddPeter J Espenshade
Mar 24, 2006·Nature·Nevan J KroganJack F Greenblatt
Jun 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael A McDonoughChristopher J Schofield
Jun 20, 2006·Genetics·Brandon S J Davies, Jasper Rine

❮ Previous
Next ❯

Citations

Sep 26, 2012·Biotechnology Letters·M Isabel González SisoM Esperanza Cerdán
Jul 27, 2012·Molecular Biology of the Cell·Joshua R PorterPablo A Iglesias
Jul 3, 2013·Annual Review of Microbiology·Geraldine Butler
Jul 13, 2012·Genetics·Roy Parker
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christoph LoenarzChristopher J Schofield
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rachelle S SingletonMatthew E Cockman
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Maximiliano J KatzPablo Wappner
May 13, 2014·Nature·Rasheduzzaman ChowdhuryChristopher J Schofield
Jan 24, 2012·Biochimie·Marc GrailleValérie Heurgué-Hamard
Sep 15, 2015·Biochemistry·James E LongbothamNigel S Scrutton
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·John S ScottiChristopher J Schofield
Sep 8, 2017·Metallomics : Integrated Biometal Science·Sergi PuigMaría Teresa Martínez-Pastor
Jan 18, 2017·The EMBO Journal·Asier González, Michael N Hall
Nov 9, 2014·The Journal of Biological Chemistry·Gururaj ShivangeRoy Anindya
Feb 13, 2018·Chemical Science·Tzu-Lan YehChristopher J Schofield
Jul 20, 2019·The Journal of Biological Chemistry·Suzanne J AdmiraalPatrick J O'Brien
Aug 31, 2017·Seminars in Cell & Developmental Biology·Risa Burr, Peter J Espenshade
Jun 3, 2021·Microorganisms·Antonia María RomeroSergi Puig

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.