PMID: 9425125Feb 28, 1998Paper

Structural and functional properties of the N transcriptional activation domain of thyroid transcription factor-1: similarities with the acidic activation domains

The Biochemical Journal
G TellG Damante

Abstract

The thyroid transcription factor 1 (TTF-1) is a tissue-specific transcription factor involved in the development of thyroid and lung. TTF-1 contains two transcriptional activation domains (N and C domain). The primary amino acid sequence of the N domain does not show any typical characteristic of known transcriptional activation domains. In aqueous solution the N domain exists in a random-coil conformation. The increase of the milieu hydrophobicity, by the addition of trifluoroethanol, induces a considerable gain of alpha-helical structure. Acidic transcriptional activation domains are largely unstructured in solution, but, under hydrophobic conditions, folding into alpha-helices or beta-strands can be induced. Therefore our data indicate that the inducibility of alpha-helix by hydrophobic conditions is a property not restricted to acidic domains. Co-transfections experiments indicate that the acidic domain of herpes simplex virus protein VP16 (VP16) and the TTF-1 N domain are interchangeable and that a chimaeric protein, which combines VP16 linked to the DNA-binding domain of TTF-1, undergoes the same regulatory constraints that operate for the wild-type TTF-1. In addition, we demonstrate that the TTF-1 N domain possesses two ...Continue Reading

Citations

Sep 27, 2006·Proteins·Mi-Kyung YoonByong-Seok Choi
Oct 23, 2001·Human Mutation·A V D'EliaG Damante
Mar 30, 2001·The Journal of Biological Chemistry·J G Tang, H P Koeffler
Feb 19, 2004·The Journal of Biological Chemistry·Kwon-Sik ParkMariastella Zannini
Dec 4, 2003·Molecular and Cellular Biology·Cindy J BachurskiDihua Hong
Feb 15, 2011·The Journal of Biological Chemistry·Johnny HabchiSonia Longhi
Mar 4, 2011·Molecular Biology Reports·Cinzia PuppinGiuseppe Damante
Jan 12, 2001·American Journal of Respiratory Cell and Molecular Biology·Y S YangJ C Weissler
Mar 15, 2008·Development·Tonggong DuStewart A Anderson

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