Structural and functional role of the PsbH protein in resistance to light stress in Synechocystis PCC 6803

Functional Plant Biology : FPB
E BergantinoG M Giacometti

Abstract

Four mutants of the cyanobacterium Synechocystis sp. PCC 6803, carrying a modified PsbH subunit on a PSI-less background, were characterized by optically-detected magnetic resonance (ODMR), electron transport kinetics, and oxygen-evolving activity. Their relative tolerance to light stress was measured. Results indicate that: (i) the PsbH protein is deeply involved in determining structural and functional properties of the QB site on the D1 protein, whereas the environment of the primary donor P680 and its acceptors pheophytin and QA are not significantly affected by modifications of this subunit or its deletion; (ii) the charge recombination rate, in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), is reduced by a factor of 2, independently of the particular modification. The same result is found with the strain in which the subunit has been deleted. This result is taken as an indication that PsbH is important in regulating protein dynamics of the entire PSII core complex; (iii)all investigated mutants display reduced tolerance to light stress, the extent of which depends on the particular modification. In this respect, mutations introduced in the transmembrane portion of the polypeptide are more effective than t...Continue Reading

Citations

Aug 12, 2003·The Journal of Biological Chemistry·Elisabetta BergantinoGiorgio Mario Giacometti

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