PMID: 8955886Nov 1, 1996Paper

Structural and functional roles of the amino-terminal region and collagen-like domain of human serum mannan-binding protein

Biochemistry and Molecular Biology International
Y MaT Kawasaki

Abstract

The serum mannan-binding protein (S-MBP) is a Ca(2+)-dependent C-type animal lectin specific for mannose and N-acetylglucosamine, which plays an important role in first-line host defense. To study the structure and function relationship of the lectin, a full-length human S-MBPcDNA was expressed in Sf9 insect cells using a baculovirus expression system, and a cDNA encoding the carbohydrate recognition domain (CRD) of human S-MBP was expressed in E. coli. The properties of the recombinant S-MBP and recombinant S-MBP-CRD were compared with those of the native human S-MBP and the CRD of the native S-MBP. The results indicated that functional human S-MBP can be successfully expressed in Sf9 cells and functional S-MBP-CRD in E. coli. In addition, the amino-terminal region and collagen-like domain are required for higher oligomer formation and play important roles in complement activation.

Citations

May 19, 2001·International Immunopharmacology·T Vorup-JensenJ C Jensenius
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y MaT Kawasaki
Dec 4, 2012·Protein Expression and Purification·Byung Cheol AhnJaeseung Yoon
Jun 16, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·T Vorup-JensenT G Jensen
Nov 9, 2016·Tissue Engineering. Part B, Reviews·Cyril AddiGregory De Crescenzo

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