Structural and functional studies of the response regulator HupR

Journal of Molecular Biology
Karen M DaviesCatherine Vénien-Bryan

Abstract

HupR is a response regulator that controls the synthesis of the membrane-bound [NiFe]hydrogenase of the photosynthetic bacterium Rhodobacter capsulatus. The protein belongs to the NtrC subfamily of response regulators and is the second protein of a two-component system. We have crystallized the full-length protein HupR in the unphosphorylated state in two dimensions using the lipid monolayer technique. The 3D structure of negatively stained HupR was calculated to a resolution of approximately 23 A from tilted electron microscope images. HupR crystallizes as a dimer, and forms an elongated V-shaped structure with extended arms. The dimensions of the dimer are about 80 A length, 40 A width and 85 A thick. The HupR monomer consists of three domains, N-terminal receiver domain, central domain and C-terminal DNA-binding domain. We have fitted the known 3D structure of the central domain from NtrC1 Aquifex aeolicus protein into our 3D model; we propose that contact between the dimers is through the central domain. The N-terminal domain is in contact with the lipid monolayer and is situated on the top of the V-shaped structure. The central domain alone has been expressed and purified; it forms a pentamer in solution and lacks ATPase a...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·V WeissB Magasanik
Jan 1, 1992·Annual Review of Genetics·J S Parkinson, E C Kofoid
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Jan 1, 1982·Progress in Biophysics and Molecular Biology·L A AmosP N Unwin
Jan 1, 1996·Journal of Structural Biology·R A CrowtherJ M Smith
Jul 14, 1998·Antimicrobial Agents and Chemotherapy·J F Barrett, J A Hoch
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·O Lenz, B Friedrich
Oct 8, 1999·Journal of Molecular Biology·J G PeltonD E Wemmer
Mar 15, 2000·Journal of Molecular Biology·C Vénien-BryanS Courty
Jul 6, 2000·Nature Structural Biology·J D Gralla
Jul 13, 2000·The Journal of Cell Biology·R D Vale
Aug 26, 2000·Journal of Bacteriology·J LeeS Kustu
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M G MeyerB T Nixon
Jul 27, 2001·Journal of Structural Biology·W Wriggers, S Birmanns
Jul 28, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Ogura, A J Wilkinson
Dec 26, 2001·Current Opinion in Structural Biology·H S ChoD E Wemmer
May 9, 2002·Biochimica Et Biophysica Acta·Alan E SeniorJoachim Weber
Oct 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sungdae ParkB Tracy Nixon
Jul 30, 2003·Biochemistry·Curtis A HastingsDavid E Wemmer
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Mar 26, 2005·Science·Mathieu RappasXiaodong Zhang
Jul 12, 2005·Journal of Structural Biology·László Sallai, Paul A Tucker

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Citations

Nov 4, 2008·Journal of Molecular Biology·Karen M DaviesLouise N Johnson
May 9, 2015·Photosynthesis Research·David Gonzalez-BallesterEmilio Fernandez
Jul 16, 2010·FEMS Microbiology Reviews·Tamaswati GhoshXiaodong Zhang
Oct 12, 2007·Chemical Reviews·Paulette M Vignais, Bernard Billoud

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