Structural and Functional Studies on the Marburg Virus GP2 Fusion Loop

The Journal of Infectious Diseases
Nina LiuJonathan R Lai

Abstract

Marburg virus (MARV) and the ebolaviruses belong to the family Filoviridae (the members of which are filoviruses) that cause severe hemorrhagic fever. Infection requires fusion of the host and viral membranes, a process that occurs in the host cell endosomal compartment and is facilitated by the envelope glycoprotein fusion subunit, GP2. The N-terminal fusion loop (FL) of GP2 is a hydrophobic disulfide-bonded loop that is postulated to insert and disrupt the host endosomal membrane during fusion. Here, we describe the first structural and functional studies of a protein corresponding to the MARV GP2 FL. We found that this protein undergoes a pH-dependent conformational change, as monitored by circular dichroism and nuclear magnetic resonance. Furthermore, we report that, under low pH conditions, the MARV GP2 FL can induce content leakage from liposomes. The general aspects of this pH-dependent structure and lipid-perturbing behavior are consistent with previous reports on Ebola virus GP2 FL. However, nuclear magnetic resonance studies in lipid bicelles and mutational analysis indicate differences in structure exist between MARV and Ebola virus GP2 FL. These results provide new insight into the mechanism of MARV GP2-mediated cel...Continue Reading

References

Oct 26, 1976·Biochimica Et Biophysica Acta·C Tanford, J A Reynolds
May 21, 1974·Biochemistry·C TanfordS Makino
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·V N MalashkevichP S Kim
Jun 8, 2001·Annual Review of Biochemistry·D M Eckert, P S Kim
Apr 16, 2005·Science·Kartik ChandranJames M Cunningham
Nov 8, 2006·Protein Science : a Publication of the Protein Society·Joseph A MarshJulie D Forman-Kay
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Jul 4, 2008·Nature Structural & Molecular Biology·Stephen C Harrison
Jun 30, 2009·Current Opinion in Structural Biology·Jeffrey E Lee, Erica Ollmann Saphire
Oct 16, 2010·Journal of the American Chemical Society·Ivan V KorendovychWilliam F Degrado
Jan 29, 2011·Journal of Virology·Arnab BasuTerry L Bowlin
Apr 2, 2011·The Journal of Biological Chemistry·Emily Happy MillerKartik Chandran
May 4, 2011·Angewandte Chemie·Krzysztof Kazimierczuk, Vladislav Yu Orekhov
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sonia M GregoryLukas K Tamm
Jul 9, 2011·Protein Science : a Publication of the Protein Society·Joseph S HarrisonJonathan R Lai
Aug 26, 2011·Nature·Jan E CaretteThijn R Brummelkamp
Mar 27, 2012·Current Opinion in Virology·Emily Happy Miller, Kartik Chandran
May 17, 2012·Viruses·Shridhar BaleErica Ollmann Saphire
Sep 1, 2012·Biochemistry·Jayne F KoellhofferJonathan R Lai
Nov 6, 2013·Immunotherapy·Erica Ollmann Saphire
Apr 4, 2014·Journal of Virology·Sonia M GregoryLukas K Tamm

❮ Previous
Next ❯

Related Concepts

Related Feeds

Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.