PMID: 2502169May 2, 1989Paper

Structural and functional studies on the sodium- and chloride-coupled gamma-aminobutyric acid transporter: deglycosylation and limited proteolysis

Biochemistry
B I KannerR Radian

Abstract

The sodium- and chloride-coupled gamma-aminobutyric transporter, an 80-kDa glycoprotein, has been subjected to deglycosylation and limited proteolysis. The treatment of the 80-kDa band with endoglycosidase F results in its disappearance and reveals the presence of a polypeptide with an apparent molecular mass of about 60 kDa, which is devoid of 125I-labeled wheat germ agglutinin binding activity but is nevertheless recognized by the antibodies against the 80-kDa band. Upon limited proteolysis with papain or Pronase, the 80-kDa band was degraded to one with an apparent molecular mass of about 60 kDa. This polypeptide still contains the 125I-labeled wheat germ agglutinin binding activity but is not recognized by the antibody. The effect of proteolysis on function was examined. The transporter was purified by use of all steps except that for the lectin chromatography [Radian, R., Bendahan, A., & Kanner, B.I. (1986) J. Biol. Chem. 261, 15437-15441]. After papain treatment and lectin chromatography, gamma-aminobutyric transport activity was eluted with N-acetylglucosamine. The characteristics of transport were the same as those of the pure transporter, but the preparation contained instead of the 80-kDa polypeptide two fragments of ...Continue Reading

References

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Citations

Jun 18, 1991·Biochimica Et Biophysica Acta·M KnipperH Breer
Dec 1, 1990·Trends in Neurosciences·M J Kuhar
Jul 1, 1992·Trends in Neurosciences·G R Uhl
Jun 1, 1991·Current Opinion in Neurobiology·S G Amara, T Pacholczyk
Jun 1, 1993·Current Opinion in Neurobiology·J Alder, M M Poo
Aug 1, 1989·Current Opinion in Cell Biology·B I Kanner
Sep 13, 1991·Biochimica Et Biophysica Acta·H T McMahon, D G Nicholls
Oct 28, 1998·Physiological Reviews·M PalacínA Zorzano
Jan 10, 1997·The Journal of Biological Chemistry·E R Bennett, B I Kanner
Sep 14, 2019·Behavioural Brain Research·Ewa Siucinska
Feb 23, 2019·Neuropharmacology·Oshrat Dayan-Alon, Baruch I Kanner
Sep 14, 1990·Science·J GuastellaB I Kanner

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