Structural and functional studies on Ycf12 (Psb30) and PsbZ-deletion mutants from a thermophilic cyanobacterium

Biochimica Et Biophysica Acta
Kenji TakasakaJian-Ren Shen

Abstract

Ycf12 (Psb30) and PsbZ are two low molecular weight subunits of photosystem II (PSII), with one and two trans-membrane helices, respectively. In order to study the functions of these two subunits from a structural point of view, we constructed deletion mutants lacking either Ycf12 or PsbZ from Thermosynechococcus elongatus, and purified, crystallized and analyzed the structure of PSII dimer from the two mutants. Our results showed that Ycf12 is located in the periphery of PSII, close to PsbK, PsbZ and PsbJ, and corresponded to the unassigned helix X1 reported previously, in agreement with the recent structure at 2.9 A resolution (A. Guskov, J. Kern, A. Gabdulkhakov, M. Broser, A. Zouni, W. Saenger, Cyanobacterial photosystem II at 2.9 A resolution: role of quinones, lipids, channels and chloride, Nat. Struct. Mol. Biol. 16 (2009) 334-342). On the other hand, crystals of PsbZ-deleted PSII showed a remarkably different unit cell constants from those of wild-type PSII, indicating a role of PsbZ in the interactions between PSII dimers within the crystal. This is the first example for a different arrangement of PSII dimers within the cyanobacterial PSII crystals. PSII dimers had a lower oxygen-evolving activity from both mutants tha...Continue Reading

References

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Citations

Feb 19, 2013·Photosynthesis Research·Cristina PaglianoJames Barber
Mar 2, 2011·Journal of Photochemistry and Photobiology. B, Biology·Natsuko Inoue-KashinoYuichiro Takahashi
Sep 13, 2011·Biochimica Et Biophysica Acta·Lan-Xin ShiWolfgang P Schröder
Jun 1, 2016·Frontiers in Plant Science·Daniel A WeiszHimadri B Pakrasi
Mar 27, 2010·Annals of Botany·Peter J NixonJosef Komenda
Mar 31, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Albert GuskovAthina Zouni
Aug 21, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Shuhei KoedaToshihisa Mizuno
Oct 21, 2016·Bioconjugate Chemistry·Masahide ShibataToshihisa Mizuno

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