PMID: 3758079Sep 15, 1986Paper

Structural and immunological comparison of human thrombospondins isolated from platelets and from culture supernatants of endothelial cells and fibroblasts. Evidence for a thrombospondin polymorphism

European Journal of Biochemistry
P ClezardinJ Dawes

Abstract

Thrombospondin is a 450-kDa glycoprotein secreted by a variety of cells including endothelial cells, fibroblasts and platelets. The aim of this study was to compare the structural and immunological properties of human endothelial, fibroblast and platelet thrombospondins. All three thrombospondins were purified, digested with thermolysin, and the subsequent thermolysin-generated fragments isolated on a Superose 12 gel-permeation column using non-denaturating conditions. Each isolated proteolytic fragment of thrombospondins was then detected using either a radioimmunoassay with a polyclonal antibody or an enzyme-linked immunosorbent assay with three monoclonal antibodies (P10, MA-I, MA-II) directed against different epitopes of whole platelet thrombospondin. The fragmentation pattern of human endothelial thrombospondin consists of six major thermolysin-generated fragments (135-110, 98-82, 54-47, 25-20, 18-15 and 10 kDa) having molecular masses very similar to those observed with human fibroblast thrombospondin (115-100, 92-80, 54-49, 27-21, 17-13 and 12-10 kDa). Treatment of platelet thrombospondin with thermolysin only generated four proteolytic fragments having molecular masses of 110, 50, 25 and 12/10 kDa respectively. All the...Continue Reading

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Citations

Oct 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P ClezardinC Kaplan
Oct 15, 1987·European Journal of Biochemistry·R Dardik, J Lahav
Aug 1, 1988·European Journal of Biochemistry·P ClezardinJ L McGregor
Jul 1, 1993·In Vitro Cellular & Developmental Biology. Animal·V MorandiY J Legrand
Mar 6, 2008·Vascular and Endovascular Surgery·Xiu-Jie WangVivian Gahtan
Apr 1, 1989·Archives of Biochemistry and Biophysics·K FurukawaA Kobata

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