Structural and kinetic characterizations of the polysialic acid O-acetyltransferase OatWY from Neisseria meningitidis.

The Journal of Biological Chemistry
Ho Jun LeeNatalie C J Strynadka


The neuroinvasive pathogen Neisseria meningitidis has 13 capsular serogroups, but the majority of disease is caused by only 5 of these. Groups B, C, Y, and W-135 all display a polymeric sialic acid-containing capsule that provides a means for the bacteria to evade the immune response during infection by mimicking host sialic acid-containing cell surface structures. These capsules in serogroups C, Y, and W-135 can be further acetylated by a sialic acid-specific O-acetyltransferase, a modification that correlates with decreased immunoreactivity and increased virulence. In N. meningitidis serogroup Y, the O-acetylation reaction is catalyzed by the enzyme OatWY, which we show has clear specificity toward the serogroup Y capsule ([Glc-(alpha1-->4)-Sia](n)). To understand the underlying molecular basis of this process, we have performed crystallographic analysis of OatWY with bound substrate as well as determined kinetic parameters of the wild type enzyme and active site mutants. The structure of OatWY reveals an intimate homotrimer of left-handed beta-helix motifs that frame a deep active site cleft selective for the polysialic acid-bearing substrate. Within the active site, our structural, kinetic, and mutagenesis data support the ...Continue Reading


Mar 1, 1979·The Journal of Experimental Medicine·F OrskovJ B Robbins
Feb 1, 1992·Glycobiology·F A Troy
Jan 21, 1997·Biochemistry·T W BeamanS L Roderick
May 20, 1998·Biochimie·A Klein, P Roussel
Oct 13, 1999·FEMS Immunology and Medical Microbiology·M L Kim, J M Slauch
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Apr 9, 2002·Structure·Xing-Guo WangSteven L Roderick
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider, George M Sheldrick
Dec 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas C Terwilliger
May 29, 2003·The Journal of Biological Chemistry·Laura E KehoeIain A Murray
Dec 4, 2003·Molecular Microbiology·Heike ClausUlrich Vogel
Feb 21, 2004·Nature Reviews. Neuroscience·Ralf Kleene, Melitta Schachner
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Eric R VimrSusan M Steenbergen
Jul 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Amanda L LewisAjit Varki
Jul 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Alexander W Schüttelkopf, Daan M F van Aalten
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Apr 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Eric L DeszoEric R Vimr
Mar 15, 2006·Structure·Prathima IengarPadmanabhan Balaram
Mar 23, 2006·Acta Crystallographica. Section D, Biological Crystallography·Jay Painter, Ethan A Merritt
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Allison H WilliamsChristian R H Raetz
Dec 6, 2006·Biochemistry·Ae-Ri KimBrian H Shilton
Jan 20, 2007·FEMS Microbiology Reviews·David S Stephens
Mar 23, 2007·Clinical and Vaccine Immunology : CVI·Peter C FuscoFrancis Michon
Apr 25, 2007·Nucleic Acids Research·Ian W DavisDavid C Richardson
May 24, 2007·The Journal of Biological Chemistry·Anne K BergfeldMartina Mühlenhoff
Feb 15, 2008·The Journal of Infectious Diseases·Martin C J MaidenJames M Stuart
Mar 17, 2009·Nature Reviews. Microbiology·Mumtaz Virji
Mar 22, 2013·MMWR. Recommendations and Reports : Morbidity and Mortality Weekly Report. Recommendations and Reports·Amanda C CohnUNKNOWN Centers for Disease Control and Prevention (CDC)
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯


Mar 30, 2010·Applied Microbiology and Biotechnology·Miguel Angel Ferrero, Leandro Rodríguez Aparicio
Oct 16, 2010·Glycobiology·Sigrid ArmingReinhard Vlasak
Mar 11, 2011·PloS One·Eike C SchulzMartina Mühlenhoff
Jun 20, 2015·Critical Reviews in Microbiology·Yih-Ling TzengDavid S Stephens
Mar 3, 2018·Vaccines·Pumtiwitt C McCarthyLaleh Sheikhi Moghaddam
Sep 20, 2020·Nature Communications·Timm FiebigMartina Mühlenhoff
Jul 30, 2020·Scientific Reports·Hugo F AzurmendiWillie F Vann
May 7, 2015·Acta Crystallographica. Section D, Biological Crystallography·Alice NgoAndrew J Fisher
Jun 23, 2021·The Journal of Biological Chemistry·Eline A VisserChristian Büll
Sep 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Carys S JonesAnthony J Clarke

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.