PMID: 8994873Dec 1, 1996Paper

Structural and mechanistic studies of enolase

Current Opinion in Structural Biology
G H ReedI Rayment

Abstract

The high-resolution structure of yeast enolase cocrystallized with its equilibrium mixture of substrate and product reveals the stereochemistry of substrate/product binding and therefore the groups responsible for acid/base catalysis and stabilization of the enolate intermediate. Expression and characterization of site-specific mutant forms of the enzyme have confirmed the roles of amino acid side chains in the catalysis of the first and second steps of the reaction. Coordination of both required magnesium ions to the carboxylate of the substrate/product indicates a role for these cations in stabilization of the intermediate.

References

Aug 23, 1977·Biochemistry·L D FallerR X Ewall
Dec 1, 1966·Analytical Chemistry·J B Terrill, C N Reilley
Jun 5, 1984·Biochemistry·V E AndersonW W Cleland
Jan 1, 1981·CRC Critical Reviews in Biochemistry·J M Brewer
Oct 3, 1995·Biochemistry·S DuquerroyJ Janin
Aug 16, 1995·Biochimica Et Biophysica Acta·V S SangadalaJ M Brewer
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy

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Citations

Sep 23, 2003·Journal of Molecular Biology·Michael SilbersteinSandor Vajda
Mar 21, 2007·Journal of the American Chemical Society·Raanan CarmieliDaniella Goldfarb
Jun 27, 2002·European Journal of Biochemistry·Luis Fernando García-Alles, Bernhard Erni
Jul 19, 2003·European Journal of Biochemistry·Véronique HannaertPaul A M Michels
Aug 27, 2010·The Journal of Biological Chemistry·Ryuichiro SuzukiShinya Fushinobu
Jun 24, 2011·Acta Crystallographica. Section D, Biological Crystallography·Eike C SchulzRalf Ficner
Mar 4, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Juan WangXiao Dong Su
Apr 29, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y J Zheng, T C Bruice
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M S HassonD Ringe
May 13, 2014·Nitric Oxide : Biology and Chemistry·Naihao LuYi-Yuan Peng
May 10, 2012·Molecular and Biochemical Parasitology·Araceli Castillo-RomeroFrances D Gillin
Jun 18, 2015·Frontiers in Bioengineering and Biotechnology·Oleg A ZadvornyyJohn W Peters
Jun 2, 2009·FEMS Immunology and Medical Microbiology·Masaaki MinamiHidemi Goto
Jun 30, 2005·FEMS Immunology and Medical Microbiology·Danuta WitkowskaAndrzej Gamian
Oct 29, 2014·Applied Biochemistry and Biotechnology·Karla Lizbeth Macías-SánchezMa Guadalupe Martínez-Cadena
Jul 1, 2016·Plant Physiology and Biochemistry : PPB·Vincent BillardPhilippe Etienne
Jan 19, 2010·Free Radical Biology & Medicine·Naihao LuZhonghong Gao
Oct 26, 2001·Journal of Molecular Biology·K Kühnel, B F Luisi
Apr 30, 2017·The Journal of Biological Chemistry·Joseph E WedekindJermaine L Jenkins
Apr 4, 2017·Acta Crystallographica. Section F, Structural Biology Communications·Congcong SunZhongliang Su
Oct 3, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Jadwiga PietkiewiczRegina Danielewicz
Dec 22, 2009·Molecular BioSystems·Matthias SteinRebecca C Wade
Jan 21, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Thorsten GreinertChristoph Held
Jul 4, 2007·Molecular and Biochemical Parasitology·François GuillouBenjamin E F Gourbal
Jan 5, 2021·Journal of the American Chemical Society·Emily J ThompsonJudith P Klinman
Sep 8, 2021·Journal of the American Chemical Society·Manfred T Reetz, Marc Garcia-Borràs
Jun 30, 2009·Methods : a Companion to Methods in Enzymology·Robert C Spitale, Joseph E Wedekind

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