Structural and molecular characterization of dynein in a gall-midge insect having motile sperm with only the outer arm

Cell Motility and the Cytoskeleton
P LupettiR Dallai

Abstract

The dipteran Monarthropalpus flavus possesses a peculiar sperm axoneme, characterized by multiple rows of microtubular doublets linked by the outer dynein arms only, lacking any equivalent of the central pair/radial spoke complex. The structure of these dynein molecules was studied by electron microscopy (EM). Using the quick-freeze, deep-etch method of EM, they were found to be similar to outer dynein arms described previously. Two globular "heads," each subdivided by a cleft, are clearly discernible. "Stalks" extend from proximal head to contact the B-tubule of the adjacent doublet. Unlike the situation in vertebrate sperm, the stalks sometimes branch into two thinner strands that contact the B-tubule at different sites. Treatment of demembranated sperm cells with ATP and vanadate induces conformational changes in the dynein outer arms. These are interpreted as the result of rotation of the dynein head with respect to what is observed in axonemes in rigor condition (after ATP depletion). SDS-PAGE indicates that the high-molecular-weight complement of this molecule comprises a single heavy chain. Specific dynein heavy chain-related DNA sequences corresponding to the catalytic-phosphate binding region were amplified by RT-PCR. ...Continue Reading

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Citations

Jul 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mikito OwaKen-ichi Wakabayashi
Aug 23, 2008·Arthropod Structure & Development·Eugenio PaccagniniRomano Dallai
May 29, 2007·Tissue & Cell·Romano DallaiPietro Lupetti
Apr 10, 2008·Biological Reviews of the Cambridge Philosophical Society·Michael Werner, Leigh W Simmons
Mar 24, 2009·Cell Motility and the Cytoskeleton·Maria Giovanna RiparbelliRomano Dallai
Aug 3, 2014·Journal of Proteomics·Valérie LabasElisabeth Blesbois
Apr 29, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Charles B Lindemann, Kathleen A Lesich
Nov 5, 1999·Cell Motility and the Cytoskeleton·H MohriY Yano-Toyoshima
Jun 29, 2007·Cell Motility and the Cytoskeleton·Eugenio PaccagniniRomano Dallai
Aug 18, 2005·Cell Motility and the Cytoskeleton·Pietro LupettiCaterina Mencarelli
Nov 2, 2002·Molecular Reproduction and Development·Sumio IshijimaKazuhiko Matsushita

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