Structural and ontogenetic study of the urachus in human fetuses

Cells, Tissues, Organs
Helena M F PazosLuciano A Favorito

Abstract

The objective of this work was to conduct an ontogenetic and structural study of the urachus. We studied 40 human fetuses (13-20 weeks post conception, WPC). The urachus was stained in Masson's trichrome, to quantify connective tissue and smooth muscle and to determine the urachal lumen area. Weigert's resorcin-fuchsin was used to observe elastic fibers, and picrosirius red and immunohistochemistry analysis were used to observe collagen. The images were captured with Olympus BX51 microscopy and an Olympus DP70 camera. The stereological analysis was done using the software Image Pro and Image J, to determine volumetric densities. For biochemical analysis, the collagen concentrations were expressed per milligram of dry tissue. Means were compared using the unpaired t test (p < 0.05). Quantitative analysis documented a statistically insignificant increase (p = 0.1475) in volumetric densities of smooth muscle in the urachus of males (23.02%), when compared with females (18.43%), and a statistically significant increase (p = 0.0439) in volumetric densities of connective tissue in the urachus of females, (67.64%) when compared with males (58.38%). Total collagen concentrations in the male (31,919-56.792 microg/mg, mean 45,656) and fe...Continue Reading

Citations

Nov 30, 2014·Urology·Joseph W McQuaidBartley G Cilento
Mar 10, 2015·Diagnostic and Interventional Imaging·J You-Ling ShyuA J Adduci
Apr 29, 2015·Journal of Pediatric Surgery·Alaa CheikhelardSabine Sarnacki

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