PMID: 2492937Jan 15, 1989Paper

Structural and physicochemical requirements of endotoxins for the activation of arachidonic acid metabolism in mouse peritoneal macrophages in vitro

European Journal of Biochemistry
T LüderitzE T Rietschel

Abstract

Lipopolysaccharides of different wild-type and mutant gram-negative bacteria, as well as synthetic and bacterial free lipid A, were studied for their ability to activate arachidonic acid metabolism in mouse peritoneal macrophages in vitro. It was found that lipopolysaccharides of deep-rough mutants of Salmonella minnesota and Escherichia coli (Re to Rc chemotypes) stimulated macrophages to release significant amounts of leukotriene C4 (LTC4) and prostaglandin E2 (PGE2). Lipopolysaccharides of wild-type strains (S. abortus equi, S. friedenau) only induced PGE2 and not LTC4 formation. Unexpectedly, free bacterial and synthetic E. coli lipid A were only weak inducers of LTC4 and PGE2 production. Deacylated Re-mutant lipopolysaccharide preparations were inactive. However, co-incubation of macrophages with both deacylated lipopolysaccharide and lipid A lead to the release of significant amounts of LTC4 and PGE2, similar to those obtained with Re-mutant lipopolysaccharide. The significance of the lipid A portion of lipopolysaccharide for the induction of LTC4 was indicated by demonstrating that peritoneal macrophages of endotoxin-low-responder mice or of mice rendered tolerant to endotoxin did not respond with the release of arachido...Continue Reading

References

Jan 1, 1979·Pharmacology & Therapeutics·J B Clark, J M Land
Feb 1, 1978·Infection and Immunity·G G Greer, E T Rietschel
Jun 1, 1975·European Journal of Biochemistry·C Galanos, O Lüderitz
Apr 1, 1985·European Journal of Biochemistry·C GalanosH Yoshimura
Mar 3, 1986·European Journal of Biochemistry·T LüderitzE T Rietschel
Jan 1, 1986·Microbiology and Immunology·T KirikaeO Lüderitz
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Jul 1, 1984·Reviews of Infectious Diseases·O LüderitzT Shiba
Jul 15, 1982·Klinische Wochenschrift·U Schade, E T Rietschel

❮ Previous
Next ❯

Citations

Jan 1, 1999·Chemistry and Physics of Lipids·K Brandenburg, U Seydel
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·A PoltorakB Beutler
Mar 1, 1993·Antimicrobial Agents and Chemotherapy·G MateraP V Halushka
Jun 9, 2009·Applied and Environmental Microbiology·Toru MiyamotoNoriyuki Kasai
Sep 11, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Nadia SafirLayachi Chabraoui
Nov 5, 2013·The Journal of Laryngology and Otology·M H BaradaranfarS Atighechi
Nov 30, 1999·Molecular Cell Biology Research Communications : MCBRC·C A AurellA O Wiström
Jun 15, 1990·Biochemical and Biophysical Research Communications·B A ParkarS J Foster
Mar 1, 1992·Journal of Structural Biology·K BrandenburgU Seydel
Apr 1, 1993·Immunobiology·D C MorrisonT Y Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.