Structural and segregational stability of various replicons in Actinobacillus actinomycetemcomitans

Plasmid
Dominique M GalliDonald J Leblanc

Abstract

Few vectors suitable for cloning in Actinobacillus actinomycetemcomitans, a periodontal pathogen, have been described. These plasmids were based either on the native A. actinomycetemcomitans replicon, pVT736-1, or derivatives of the IncP and IncQ family of plasmids. Their usefulness as cloning vectors is limited because of instability or size. Therefore, the ability of additional replicons to function in A. actinomycetemcomitans was evaluated. Derivatives of p15A, ColE1/f1, and pWV01 transformed A. actinomycetemcomitans efficiently and exhibited no structural instability in the new host. The stable maintenance of A. actinomycetemcomitans-specific DNA fragments inserted into the p15A derivative, pDMG4, demonstrated the ability of this plasmid to function as a cloning vector. In addition, pVT736-1 was used to clone selectable markers directly into A. actinomycetemcomitans. These constructs were structurally and segregationally stable.

Citations

Oct 16, 1999·Periodontology 2000·P M Fives-TaylorC Brissette
Feb 13, 2001·Journal of Bacteriology·D M GalliD J Leblanc
Oct 11, 2002·Journal of Bacteriology·Jinbiao ChenDominique M Galli
Apr 13, 2011·PLoS Pathogens·Matthew M RamseyMarvin Whiteley
Feb 11, 2010·Plasmid·Jinbiao ChenDominique M Galli
Sep 20, 2005·Plasmid·Dominique M Galli, Jinbiao Chen
May 2, 2002·Plasmid·Dominique M GalliDonald J LeBlanc
Oct 7, 2003·Annual Review of Microbiology·Brian HendersonMichael Wilson

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