Structural and spectroscopic consequences of hexacoordination of a bacteriochlorophyll cofactor in the Rhodobacter sphaeroides reaction center

Biochemistry
Dmitrij FrolovM R Jones

Abstract

The structural and functional consequences of changing the coordination state of one of the bacteriochlorophyll (BChl) cofactors in the purple bacterial reaction center have been explored. A combination of steady state spectroscopy and X-ray crystallography was used to demonstrate that mutagenesis of residue 181 of the L-polypeptide from Phe to Arg (FL181R) causes the BChl at the accessory (B(B)) position on the so-called inactive cofactor branch to become hexacoordinated, with no significant changes to the structure of the surrounding protein. This change was accompanied by the appearance of a distinctive absorbance band at 631 nm in the room-temperature absorbance spectrum. The ligand donor was not the Arg side chain but rather an intervening water molecule, and contrary to expectations, the Mg of B(B) did not adopt a more in-plane geometry in response to hexacoordination. The mutation caused a disturbance to the detailed conformation of the BChl macrocycle that manifested in a number of subtle changes to the resonance Raman spectrum. Hexacoordination of B(B) produced a small increase in the lifetime of the excited electronic state of the primary donor bacteriochlorophylls (P*), indicating some disturbance to light-driven ene...Continue Reading

References

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J P AllenD C Rees
Feb 1, 1994·Journal of Bioenergetics and Biomembranes·U ErmlerM Schiffer
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E van BrederodeR van Grondelle
Feb 24, 2001·Sub-cellular Biochemistry·M E van Brederode, M R Jones
May 30, 2002·Biochimica Et Biophysica Acta·Selma SchenklJosef Wachtveitl
Dec 4, 2002·Biochimica Et Biophysica Acta·Teodor Silviu BalabanValentin I Prokhorenko
Feb 7, 2004·Science·Kristina N FerreiraSo Iwata
Apr 28, 2004·FEBS Letters·Adam Ben-ShemNathan Nelson
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E J Bylina, D C Youvan
Feb 17, 2009·Nature Structural & Molecular Biology·Albert GuskovWolfram Saenger
Jun 2, 2009·Biochimica Et Biophysica Acta·Teodor Silviu BalabanAthina Zouni

❮ Previous
Next ❯

Citations

Oct 1, 2011·The Journal of Physical Chemistry. a·S R K C Sharma YamijalaSwapan K Pati
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·M M LeonovaV A Shuvalov
Aug 18, 2012·The Journal of Chemical Physics·Johan Strümpfer, Klaus Schulten
Feb 6, 2017·Biochimica Et Biophysica Acta. Bioenergetics·Rafael G SaerRobert E Blankenship
Feb 14, 2012·The Journal of Physical Chemistry. B·Bhanu NeupaneRyszard Jankowiak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.