Structural and thermodynamic behavior of eukaryotic initiation factor 4E in supramolecular formation with 4E-binding protein 1 and mRNA cap analogue, studied by spectroscopic methods

Chemical & Pharmaceutical Bulletin
X ShenT Ishida

Abstract

The structural and thermodynamic behavior of the complex formation of eIF4E with either or both mRNA cap analogue (m7GTP, m7GpppA, or m7GpppG) and 4EBP1 has been investigated by spectroscopic measurements. Although the circular dichroism (CD) spectrum of eIF4E was little affected by the association with any cap analogue, the association constant of eIF4E with m7GpppA/G, estimated from the fluorescence quenching, was about 10 times larger than that with m7GTP. The van't Hoff analyses showed that the m7GpppA/G binding is enthalpy-driven with a large negative deltaH(o), and this is in contrast with the entropy-driven binding of m7GTP, where the positive deltaS(o) is large enough to overcome an increase of deltaH(o). This different behavior obviously originates in the interaction of the second nucleotide in m7GpppA with eIF4E, suggesting the importance of the nucleotide sequence linked to the m7Gppp terminal moiety, in addition to the specific interaction with the m7G base, for the recognition of mRNA cap structure by eIF4E. On the other hand, the CD spectra indicated that the binding of 4EBP1, an endogenous eIF4E-regulatory protein without having any defined secondary structure, shifted the m7GTP- or m7GpppA/G-bound eIF4E to an ir...Continue Reading

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Citations

Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Evangelos PapadopoulosGerhard Wagner
Oct 9, 2015·Journal of Receptor and Signal Transduction Research·Ye ChengYan Wang
Dec 17, 2014·Immunological Reviews·Michael J Osborne, Katherine L B Borden
Dec 20, 2011·Journal of Molecular Biology·Nadeem SiddiquiKatherine L B Borden
Jul 14, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Toshimasa Ishida
Oct 10, 2007·Methods in Enzymology·Regina CencicJerry Pelletier
Jun 30, 2018·Cell Cycle·Ilona KocmikMarzena Jankowska-Anyszka
Oct 13, 2006·The EMBO Journal·Laurent VolponKatherine L B Borden
Jan 1, 2012·F1000Research·Mark Livingstone, Michael Bidinosti
May 26, 2020·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Aldo BarreraMarcelo López-Lastra
Aug 26, 2014·RNA·Karolina PiecykMarzena Jankowska-Anyszka

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