Structural and thermodynamic characterization of Pal, a phage natural chimeric lysin active against pneumococci.

The Journal of Biological Chemistry
Julio VareaMargarita Menéndez

Abstract

Pal amidase, encoded by pneumococcal bacteriophage Dp-1, represents one step beyond in the modular evolution of pneumococcal murein hydrolases. It exhibits the choline-binding module attaching pneumococcal lysins to the cell wall, but the catalytic module is different from those present in the amidases coded by the host or other pneumococcal phages. Pal is also an effective antimicrobial agent against Streptococcus pneumoniae that may constitute an alternative to antibiotic prophylaxis. The structural implications of Pal singular structure and their effect on the choline-amidase interactions have been examined by means of several techniques. Pal stability is maximum around pH 8.0 (Tm approximately 50.2 degrees C; DeltaHt = 183 +/- 4 kcal mol(-1)), and its constituting modules fold as two tight interacting cooperative units whose denaturation merges into a single process in the free amidase but may proceed as two well resolved events in the choline-bound state. Choline titration curves reflect low energy ligand-protein interactions and are compatible with two sets of sites. Choline binding strongly stabilizes the cell wall binding module, and the conformational stabilization is transmitted to the catalytic region. Moreover, the ...Continue Reading

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Citations

Aug 20, 2010·The Journal of Biological Chemistry·Noemí BustamanteMargarita Menéndez
Aug 1, 2008·The Journal of Biological Chemistry·Begoña MonterrosoMargarita Menéndez
Nov 26, 2010·Journal of Bacteriology·Mourad SabriSylvain Moineau
Jan 12, 2013·Current Protein & Peptide Science·Zuzanna Drulis-KawaRob Lavigne
Nov 13, 2014·Molecular Microbiology·Daniela ProençaCarlos São-José
Jun 28, 2005·Current Opinion in Microbiology·Martin J Loessner
Jan 22, 2015·Critical Reviews in Biotechnology·Lorena Rodríguez-RubioPilar García
Oct 23, 2012·Biophysical Journal·Alejandro ValbuenaMariano Carrión-Vázquez
Mar 6, 2015·Protein Engineering, Design & Selection : PEDS·Ryan D HeselpothDaniel C Nelson
Jul 5, 2016·Frontiers in Microbiology·Diana GutiérrezPilar García
Dec 1, 2017·Scientific Reports·Noemí BustamanteMargarita Menéndez
Jun 12, 2016·Applied and Environmental Microbiology·Lorena Rodríguez-RubioYves Briers
Jun 10, 2005·The Biochemical Journal·Begoña MonterrosoMargarita Menéndez
Nov 19, 2020·Biotechnology Reports·Maria Daniela SilvaSanna Sillankorva
Nov 16, 2018·Journal of the American Chemical Society·Miguel Ángel TreviñoDouglas V Laurents

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