PMID: 1127016May 1, 1975

Structural and transcriptional features of the mouse spermatid genome

The Journal of Cell Biology
A L Kierszenbaum, L L Tres

Abstract

A whole-mount electron microscope technique has allowed direct visualization of the transcription process in mouse spermatids. Thes observations have been supported by light and electron microscope autoradiographic techniques that employ [3H]uridine and [3H]arginine in attempts to clarify mechanisms of RNA synthesis and their relationship to nuclear histone changes throughout spermiogenesis. Early spermatid genomes are dispersed almost completely, whereas in later spermiogenic steps the posterior or flagellar nuclear region is readily dispersed and the anterior or subacrosomal nuclear region remains compact. Display of genome segments permits identification of regions where transcription complexes, presumably heterogeneous nuclear RNA species, are seen related to chromatin. These complexes appear as ribonucleoprotein chains, some of them of considerable length, decreasing progressively in number in late spermiogenic steps. This decrease coincides with diminishing rates of [3H]uridine incorporation. Two distinct patterns of chromatin have been identified: a beaded chromatin type associated with transcription complexes encounterd in early spermatids; and a smooth chromatin type not involved in transcriptive activity observed in a...Continue Reading

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Related Concepts

Arginine hydrochloride
Histone antigen
RNA Chemical Synthesis
Ribonucleoprotein Activity
Genome
Nucleoproteins
Complex (molecular entity)
Transcription, Genetic
Transcription Process
Aphasia, Broca

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