Structural bases for recognition of Anp32/LANP proteins.

The FEBS Journal
Cesira de ChiaraAnnalisa Pastore

Abstract

The leucine-rich repeat acidic nuclear protein (Anp32a/LANP) belongs to a family of evolutionarily-conserved phosphoproteins involved in a complex network of protein-protein interactions. In an effort to understand the cellular role, we have investigated the mode of interaction of Anp32a with its partners. As a prerequisite, we solved the structure in solution of the evolutionarily conserved N-terminal leucine-rich repeat (LRR) domain and modeled its interactions with other proteins, taking PP2A as a paradigmatic example. The interaction between the Anp32a LRR domain and the AXH domain of ataxin-1 was probed experimentally. The two isolated and unmodified domains bind with very weak (millimolar) affinity, thus suggesting the necessity either for an additional partner (e.g. other regions of either or both proteins or a third molecule) or for a post-translational modification. Finally, we identified by two-hybrid screening a new partner of the LRR domain, i.e. the microtubule plus-end tracking protein Clip 170/Restin, known to regulate the dynamic properties of microtubules and to be associated with severe human pathologies.

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Citations

May 1, 2013·Human Molecular Genetics·Ivelisse SánchezAntoni Matilla-Dueñas
Apr 25, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Lu-Yun Lian
Mar 25, 2014·Protein Science : a Publication of the Protein Society·Thuy P DaoDoug Barrick

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