Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase

Biochimica Et Biophysica Acta
Kohji YamamotoMamoru Suzuki

Abstract

Glutathione transferase (GST) catalyzes glutathione conjugation, a major detoxification pathway for xenobiotics and endogenous substances. Here, we determined the crystal structure of a Delta-class GST from Bombyx mori (bmGSTD) to examine its catalytic residues. The three-dimensional structure of bmGSTD was resolved by the molecular replacement method and refined to a resolution of 2.0Å. Structural alignment with a Delta-class GST of Anopheles gambiae indicated that bmGSTD contains 2 distinct domains (an N-terminal domain and a C-terminal domain) connected by a linker. The bound glutathione localized at the N-terminal domain. Putative catalytic residues were changed to alanine by site-directed mutagenesis, and the resulting mutants were characterized in terms of catalytic activity using glutathione and 1-chloro-2,4-dinitrobenzene, a synthetic substrate of GST. Kinetic analysis of bmGSTD mutants indicated that Ser11, Gln51, His52, Ser67, and Arg68 are important for enzyme function. These results provide structural insights into the catalysis of glutathione conjugation in B. mori by bmGSTD.

References

Jan 1, 1997·Chemical Research in Toxicology·R N Armstrong
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·A A VaguineS J Wodak
Nov 28, 2000·Acta Crystallographica. Section D, Biological Crystallography·A Vagin, A Teplyakov
Nov 30, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Marcelo Hermes-Lima, Tania Zenteno-Savín
Dec 4, 2003·Acta Crystallographica. Section D, Biological Crystallography·Liqing ChenEdward J Meehan
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jun 14, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kohji YamamotoHiroshi Fujii
Oct 18, 2005·The Biochemical Journal·Jantana Wongsantichon, Albert J Ketterman
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
Feb 8, 2007·Bioscience, Biotechnology, and Biochemistry·Kohji YamamotoKatsumi Koga
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Sep 2, 2008·Archives of Biochemistry and Biophysics·Tassanee Lerksuthirat, Albert J Ketterman
Nov 22, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Kohji YamamotoYoichi Aso
Mar 4, 2010·The Biochemical Journal·Jantana WongsantichonAlbert J Ketterman
Jan 18, 2011·Biochimica Et Biophysica Acta·Kohji YamamotoTakashi Nakashima
Jul 16, 2011·Biochimica Et Biophysica Acta·Yoshimitsu KakutaKohji Yamamoto

❮ Previous
Next ❯

Citations

May 23, 2014·PloS One·M D Tofazzal HossainKohji Yamamoto
May 3, 2014·International Journal of Molecular Sciences·Xiang TanPing Zhao
Jan 23, 2015·Bioscience, Biotechnology, and Biochemistry·Md Tofazzal Hossain, Kohji Yamamoto
Jul 22, 2016·Scientific Reports·Kohji Yamamoto, Naotaka Yamada
Nov 20, 2016·Journal of Pesticide Science·Kohji YamamotoNaotaka Yamada
Aug 8, 2019·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Yun WangJian-Hua Huang
Jan 7, 2021·The Protein Journal·Kohji Yamamoto, Aiko Hirowatari
Jun 14, 2021·Pesticide Biochemistry and Physiology·Leily DaneshianMaksymilian Chruszcz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.