Structural basis for conductance through TRIC cation channels

Nature Communications
Min SuYu-Hang Chen

Abstract

Mammalian TRICs function as K+-permeable cation channels that provide counter ions for Ca2+ handling in intracellular stores. Here we describe the structures of two prokaryotic homologues, archaeal SaTRIC and bacterial CpTRIC, showing that TRIC channels are symmetrical trimers with transmembrane pores through each protomer. Each pore holds a string of water molecules centred at kinked helices in two inverted-repeat triple-helix bundles (THBs). The pores are locked in a closed state by a hydrogen bond network at the C terminus of the THBs, which is lost when the pores assume an open conformation. The transition between the open and close states seems to be mediated by cation binding to conserved residues along the three-fold axis. Electrophysiology and mutagenesis studies show that prokaryotic TRICs have similar functional properties to those of mammalian TRICs and implicate the three-fold axis in the allosteric regulation of the channel.

References

Jan 1, 1977·Physiological Reviews·M Endo
Oct 1, 1984·Physiological Reviews·A N Martonosi
Oct 1, 1980·The Journal of General Physiology·R CoronadoC Miller
Jan 1, 1983·Molecular and Cellular Biochemistry·G Meissner
Dec 1, 1996·Journal of Molecular Graphics·O S SmartM S Sansom
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 13, 2001·Seminars in Cell & Developmental Biology·M D BootmanP Lipp
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Aug 6, 1958·The Journal of Physiology·P FATT, B L GINSBORG
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
May 28, 2005·Science·Daniel O DaleyGunnar von Heijne
Jul 6, 2007·Nature·Masayuki YazawaHiroshi Takeshima
Jan 10, 2008·Biochemical and Biophysical Research Communications·Sidney Fleischer
Jul 31, 2009·Nature·Toshimitsu KawateEric Gouaux
Oct 28, 2009·Journal of Structural and Functional Genomics·Marco PuntaBurkhard Rost
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 20, 2010·International Journal of Cell Biology·Claudia CerellaLina Ghibelli
Jul 21, 2010·Biophysical Journal·Samantha J PittRebecca Sitsapesan
Sep 23, 2010·The Journal of Biological Chemistry·Xiaoli ZhaoJianjie Ma
Oct 29, 2010·Nature·Yu-Hang ChenWayne A Hendrickson
Apr 27, 2011·The Journal of Membrane Biology·Abe L F Silverio, Milton H Saier
Jul 22, 2011·Current Opinion in Structural Biology·Vadim Cherezov
Jul 24, 2012·The Journal of Biological Chemistry·Filip Van Petegem
Mar 8, 2013·Pflügers Archiv : European journal of physiology·Elisa VenturiRebecca Sitsapesan
Jan 29, 2014·Metal Ions in Life Sciences·Marisa BriniErnesto Carafoli
Oct 18, 2014·Science·Tingting YangWayne A Hendrickson
Dec 4, 2014·Nature·Ran ZalkAndrew R Marks
Jul 23, 2015·Biophysical Journal·Antoni MatyjaszkiewiczRebecca Sitsapesan
Sep 22, 2015·Nature·Jingpeng GeMaojun Yang
Sep 24, 2016·Cell·Amédée des GeorgesJoachim Frank

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Citations

Apr 9, 2020·ELife·Janine D BrunnerStephan Schenck
Feb 17, 2019·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Hui WangYu-Hang Chen
Mar 20, 2020·Journal of Molecular Evolution·Philippe YoukharibacheRavinder Abrol
Jan 22, 2021·Pflügers Archiv : European journal of physiology·Xinyu ZhouJianjie Ma

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Datasets Mentioned

BETA
GMI1000

Methods Mentioned

BETA
protein foldings
PCR
size-exclusion chromatography
light scattering
gel-filtration
X-ray

Software Mentioned

SHELX
GraphPad
HOLE
PyMOL
Clampfit
phenix
BLAST
HKL2MAP
XDS
Prism

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