Structural basis for DNA recognition and processing by UvrB

Nature Structural & Molecular Biology
James J TruglioCaroline Kisker

Abstract

DNA-damage recognition in the nucleotide excision repair (NER) cascade is a complex process, operating on a wide variety of damages. UvrB is the central component in prokaryotic NER, directly involved in DNA-damage recognition and guiding the DNA through repair synthesis. We report the first structure of a UvrB-double-stranded DNA complex, providing insights into the mechanism by which UvrB binds DNA, leading to formation of the preincision complex. One DNA strand, containing a 3' overhang, threads behind a beta-hairpin motif of UvrB, indicating that this motif inserts between the strands of the double helix, thereby locking down either the damaged or undamaged strand. The nucleotide directly behind the beta-hairpin is flipped out and inserted into a small, highly conserved pocket in UvrB.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I HusainA Sancar
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P R CaronL Grossman
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A SancarG B Sancar
Apr 7, 1995·The Journal of Biological Chemistry·D S HsuA Sancar
Jan 1, 1996·Annual Review of Biochemistry·A Sancar
Feb 21, 1997·The Journal of Biological Chemistry·Y ZouB Van Houten
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·M MachiusJ Deisenhofer
Jun 26, 2001·Research in Microbiology·N Goosen, G F Moolenaar
Nov 1, 2001·The Journal of Biological Chemistry·Milan SkorvagaBennett Van Houten
Jun 12, 2004·The EMBO Journal·James J TruglioCaroline Kisker
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Aug 17, 2004·The Journal of Biological Chemistry·Matthew J DellaVecchiaBennett Van Houten
Oct 1, 2004·The Journal of Biological Chemistry·Milan SkorvagaBennett Van Houten
Nov 12, 2005·The Journal of Biological Chemistry·Erik MaltaNora Goosen
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Aug 13, 2013·Journal of the American Chemical Society·Paige L McKibbinSheila S David
Nov 26, 2010·Chemical Research in Toxicology·Han ZhengSuse Broyde
Dec 25, 2007·Cell Research·Wei Yang
Jan 18, 2011·Nature Structural & Molecular Biology·Marcin JaciukMarcin Nowotny
Feb 7, 2012·Nature Structural & Molecular Biology·Danaya PakotipraphaDavid Jeruzalmi
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hailin WangX Chris Le
Feb 12, 2009·The Journal of Biological Chemistry·John AtkinsonPeter McGlynn
Mar 17, 2009·The Journal of Biological Chemistry·Danaya PakotipraphaDavid Jeruzalmi
Nov 11, 2008·Nucleic Acids Research·Laura A ChristensenKaren M Vasquez
Mar 5, 2013·Cold Spring Harbor Perspectives in Biology·Caroline KiskerBennett Van Houten
Mar 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Hideshi YokoyamaYoshinori Satow
Jun 27, 2008·PLoS Biology·Stefanie C WolskiCaroline Kisker
Sep 28, 2010·Journal of Nucleic Acids·Yuqin CaiNicholas E Geacintov
Jan 15, 2011·Journal of Nucleic Acids·Bo Hang
Dec 3, 2013·DNA Repair·Yuqin CaiSuse Broyde
Feb 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nia M HainesNigel J Savery
Oct 29, 2010·Journal of Nucleic Acids·Rihito MoritaSeiki Kuramitsu
Aug 22, 2006·Nature Chemical Biology·Karsten Theis
Nov 11, 2014·International Journal of Molecular Sciences·Hideshi Yokoyama, Ryuta Mizutani
Oct 23, 2012·Trends in Biochemical Sciences·Alexandra M DeaconescuNikolaus Grigorieff
Jan 20, 2012·Current Opinion in Structural Biology·Jochen Kuper, Caroline Kisker
Sep 25, 2010·Research in Microbiology·Christophe Rouillon, Malcolm F White
Mar 11, 2008·Current Opinion in Structural Biology·Eric J Enemark, Leemor Joshua-Tor
Oct 24, 2007·DNA Repair·Nora Goosen, Geri F Moolenaar
Oct 27, 2007·Trends in Biochemical Sciences·Olivier MaillardHanspeter Naegeli
Jun 19, 2007·Trends in Microbiology·Nigel J Savery
Sep 8, 2011·Protein Science : a Publication of the Protein Society·Wei Yang
Sep 16, 2006·Molecular Cell·Jana RudolfMalcolm F White
Dec 15, 2010·Molecular Cell·Laura ManelyteNigel J Savery

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