Structural Basis for DNA Recognition by the Two-Component Response Regulator RcsB

MBio
Ekaterina V FilippovaWayne F Anderson

Abstract

RcsB is a highly conserved transcription regulator of the Rcs phosphorelay system, a complex two-component signal transduction system (N. Majdalani and S. Gottesman, Annu Rev Microbiol 59:379-405, 2005; A. J. Wolfe, Curr Opin Microbiol 13:204-209, 2010, https://doi.org/10.1016/j.mib.2010.01.002; D. J. Clarke, Future Microbiol 5:1173-1184, 2010, https://doi.org/10.2217/fmb.10.83). RcsB plays an important role in virulence and pathogenicity in human hosts by regulating biofilm formation. RcsB can regulate transcription alone or together with its auxiliary transcription regulators by forming heterodimers. This complexity allows RcsB to regulate transcription of more than 600 bacterial genes in response to different stresses (D. Wang et al., Mol Plant Microbe Interact 25:6-17, 2012, https://doi.org/10.1094/MPMI-08-11-0207). Despite increasing knowledge of RcsB importance, molecular mechanisms that drive the ability of RcsB to control transcription of a large number of genes remain unclear. Here, we present crystal structures of unphosphorylated RcsB in complex with the consensus DNA-binding sequence of 22-mer (DNA22) and 18-mer (DNA18) of the flhDC operon from Escherichia coli determined at 3.15- and 3.37-Å resolution, respectively...Continue Reading

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Citations

May 19, 2019·Annual Review of Microbiology·Rong GaoAnn M Stock
Feb 28, 2021·Nucleic Acids Research·Juanjo HuesaPatricia Casino
Mar 20, 2021·Scientific Reports·Kamonlapat SupimonPa-Thai Yenchitsomanus
Mar 23, 2021·Critical Reviews in Microbiology·Lucindo Cardoso de PinaL Caetano M Antunes
Mar 24, 2021·Journal of Bacteriology·Nickolas G KesslerSean D Moore
Oct 26, 2021·Journal of Bacteriology·Yuki YamanakaKaneyoshi Yamamoto

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Methods Mentioned

BETA
two hybrid
phosphotransferase
size
light scattering
X-ray
electrophoretic mobility shift assay
electrophoresis
Surface plasmon resonance
chip

Software Mentioned

TraceDrawer
Dali

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