Structural basis for metallic-like conductivity in microbial nanowires

MBio
Nikhil S MalvankarDerek R Lovley

Abstract

Direct measurement of multiple physical properties of Geobacter sulfurreducens pili have demonstrated that they possess metallic-like conductivity, but several studies have suggested that metallic-like conductivity is unlikely based on the structures of the G. sulfurreducens pilus predicted from homology models. In order to further evaluate this discrepancy, pili were examined with synchrotron X-ray microdiffraction and rocking-curve X-ray diffraction. Both techniques revealed a periodic 3.2-Å spacing in conductive, wild-type G. sulfurreducens pili that was missing in the nonconductive pili of strain Aro5, which lack key aromatic acids required for conductivity. The intensity of the 3.2-Å peak increased 100-fold when the pH was shifted from 10.5 to 2, corresponding with a previously reported 100-fold increase in pilus conductivity with this pH change. These results suggest a clear structure-function correlation for metallic-like conductivity that can be attributed to overlapping π-orbitals of aromatic amino acids. A homology model of the G. sulfurreducens pilus was constructed with a Pseudomonas aeruginosa pilus model as a template as an alternative to previous models, which were based on a Neisseria gonorrhoeae pilus structure...Continue Reading

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Citations

Oct 18, 2015·FEMS Microbiology Reviews·Mark DopsonTom H J A Sleutels
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Dec 3, 2016·Microbiology·Sandeep SureMandira Kochar
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Jan 6, 2018·Reports on Progress in Physics·Christopher D BostickDavid Lederman
Sep 6, 2017·The ISME Journal·David Jf WalkerDerek R Lovley
Jul 14, 2016·Small·Yang TanDerek R Lovley
Aug 2, 2018·The FEBS Journal·Vipul SolankiKrishan Gopal Thakur
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Aug 5, 2020·Journal of Bacteriology·Derek R Lovley, Dawn E Holmes
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Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Poorna SubramanianGrant J Jensen
May 29, 2018·Microbial Biotechnology·Gemma Reguera
Dec 14, 2018·Applied Microbiology and Biotechnology·Xi LiuLuyan Z Ma
Apr 18, 2019·MBio·David J F WalkerDerek R Lovley
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Methods Mentioned

BETA
nuclear magnetic resonance
X-ray
transmission electron microscopy
circular dichroism
NMR
atomic force microscopy

Software Mentioned

FirstGlance
ConSurf
MolProbity
Conformer
Jmol
IGOR Pro
FIT2D
DeepView
Pdb Viewer ( DeepView )
Protein Calculator

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