Structural Basis for Oxygen Activation at a Heterodinuclear Manganese/Iron Cofactor.

The Journal of Biological Chemistry
Julia J GrieseMartin Högbom

Abstract

Two recently discovered groups of prokaryotic di-metal carboxylate proteins harbor a heterodinuclear Mn/Fe cofactor. These are the class Ic ribonucleotide reductase R2 proteins and a group of oxidases that are found predominantly in pathogens and extremophiles, called R2-like ligand-binding oxidases (R2lox). We have recently shown that the Mn/Fe cofactor of R2lox self-assembles from Mn(II) and Fe(II) in vitro and catalyzes formation of a tyrosine-valine ether cross-link in the protein scaffold (Griese, J. J., Roos, K., Cox, N., Shafaat, H. S., Branca, R. M., Lehtiö, J., Gräslund, A., Lubitz, W., Siegbahn, P. E., and Högbom, M. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 17189-17194). Here, we present a detailed structural analysis of R2lox in the nonactivated, reduced, and oxidized resting Mn/Fe- and Fe/Fe-bound states, as well as the nonactivated Mn/Mn-bound state. X-ray crystallography and x-ray absorption spectroscopy demonstrate that the active site ligand configuration of R2lox is essentially the same regardless of cofactor composition. Both the Mn/Fe and the diiron cofactor activate oxygen and catalyze formation of the ether cross-link, whereas the dimanganese cluster does not. The structures delineate likely routes for gat...Continue Reading

References

Jul 5, 1993·Journal of Molecular Biology·P Nordlund, H Eklund
Dec 1, 1995·Current Opinion in Structural Biology·P Nordlund, H Eklund
Mar 1, 1997·Protein Science : a Publication of the Protein Society·N ElangoD H Ohlendorf
Jul 15, 1995·Physical Review. B, Condensed Matter·S I ZabinskyM J Eller
May 24, 2001·Biophysical Journal·D I SvergunM H Koch
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Mar 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Martin HögbomFriedhelm Lendzian
Mar 29, 2003·Journal of Molecular Evolution·Eugenio NotomistaAnna Tramontano
Apr 26, 2003·Current Opinion in Chemical Biology·JoAnne Stubbe
May 15, 2003·The Journal of Biological Chemistry·Jonathan P McHughSimon C Andrews
Dec 18, 2003·Journal of the American Chemical Society·Walter C VoegtliAmy C Rosenzweig
Jun 25, 2004·Nucleic Acids Research·Todd J DolinskyNathan A Baker
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jun 8, 2006·Annual Review of Biochemistry·Pär Nordlund, Peter Reichard
Jul 27, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Elizabeth C TheilXiaofeng Liu
Aug 16, 2006·Accounts of Chemical Research·Matthew H Sazinsky, Stephen J Lippard
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lucas J BaileyBrian G Fox
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Charlotta S Andersson, Martin Högbom
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 13, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Martin Högbom
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Jul 31, 2010·Science·Andreas SchirmerStephen B del Cardayre

❮ Previous
Next ❯

Citations

Jul 6, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Andrew J JasniewskiLawrence Que
Jul 5, 2017·Journal of the American Chemical Society·Andrew J JasniewskiLawrence Que
May 4, 2016·Journal of Inorganic Biochemistry·Yuri KutinNicholas Cox
Oct 9, 2019·The Journal of Biological Chemistry·Yury KutinJulia J Griese
Apr 17, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kristīne GrāveMartin Högbom
Jun 28, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Julia J GrieseMartin Högbom
Jan 29, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Julia J GrieseMartin Högbom
Aug 3, 2019·Acta Crystallographica. Section D, Structural Biology·Julia J Griese, Martin Högbom
Aug 15, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kristīne GrāveMartin Högbom
Feb 3, 2021·Journal of the American Chemical Society·Kelsey R MillerA S Borovik
Feb 18, 2020·Journal of the American Chemical Society·Effie C KisgeropoulosHannah S Shafaat
Dec 23, 2017·Journal of the American Chemical Society·Pearson T MaugeriHannah S Shafaat

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Julia J GrieseMartin Högbom
Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Martin Högbom
Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Katarina Roos, Per E M Siegbahn
© 2021 Meta ULC. All rights reserved