Structural basis for specific inhibition of the highly sensitive ShHTL7 receptor

EMBO Reports
Umar Shahul HameedStefan T Arold

Abstract

Striga hermonthica is a root parasitic plant that infests cereals, decimating yields, particularly in sub-Saharan Africa. For germination, Striga seeds require host-released strigolactones that are perceived by the family of HYPOSENSITIVE to LIGHT (ShHTL) receptors. Inhibiting seed germination would thus be a promising approach for combating Striga However, there are currently no strigolactone antagonists that specifically block ShHTLs and do not bind to DWARF14, the homologous strigolactone receptor of the host. Here, we show that the octyl phenol ethoxylate Triton X-100 inhibits S. hermonthica seed germination without affecting host plants. High-resolution X-ray structures reveal that Triton X-100 specifically plugs the catalytic pocket of ShHTL7. ShHTL7-specific inhibition by Triton X-100 demonstrates the dominant role of this particular ShHTL receptor for Striga germination. Our structural analysis provides a rationale for the broad specificity and high sensitivity of ShHTL7, and reveals that strigolactones trigger structural changes in ShHTL7 that are required for downstream signaling. Our findings identify Triton and the related 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]acetic acid as promising lead compounds for the ratio...Continue Reading

References

Aug 12, 2008·Nature·Mikihisa UmeharaShinjiro Yamaguchi
Aug 12, 2008·Nature·Victoria Gomez-RoldanSoizic F Rochange
Mar 21, 2009·Pest Management Science·Sarah J Hearne
Aug 7, 2010·Annual Review of Phytopathology·Xiaonan XieKoichi Yoneyama
Mar 17, 2012·Science·Adrian AlderSalim Al-Babili
Apr 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yongxia GuoJoseph P Noel
Aug 7, 2013·Plant Methods·Jean-Bernard PouvreauPhilippe Simier
Oct 18, 2013·Nature Communications·Hidemitsu NakamuraTadao Asami
Dec 18, 2013·Nature·Liang JiangJiayang Li
Jan 27, 2015·Methods in Molecular Biology·Melody Y PaiJing Huang
Jan 27, 2015·Annual Review of Plant Biology·Salim Al-Babili, Harro J Bouwmeester
Jan 7, 2016·Pest Management Science·Binne ZwanenburgChinnaswamy Kannan
Apr 30, 2016·Annual Review of Plant Biology·Satoko YoshidaKen Shirasu
Jul 19, 2016·Nature Chemical Biology·Duncan Holbrook-SmithPeter McCourt
Aug 2, 2016·Nature·Ruifeng YaoDaoxin Xie
Aug 2, 2016·Nature Chemical Biology·Alexandre de Saint GermainFrançois-Didier Boyer
May 18, 2017·Nature Chemical Biology·Shelley LumbaPeter McCourt
Aug 16, 2017·Journal of Applied Crystallography·D FrankeD I Svergun
Dec 19, 2017·Journal of Experimental Botany·Kun-Peng JiaSalim Al-Babili
Jan 5, 2018·Journal of Experimental Botany·Muhammad JamilSalim Al-Babili

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Citations

Jul 10, 2019·Angewandte Chemie·Harro J BouwmeesterAlain De Mesmaeker
Oct 1, 2020·Plant Direct·Marco Bürger, Joanne Chory
Apr 10, 2020·Annals of Botany·Igor CesarinoMarc Somssich
Apr 2, 2021·Plant Physiology·David C Nelson
Jul 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Amir Arellano-SaabShelley Lumba
Aug 14, 2020·ACS Synthetic Biology·Rebecca J ChesterfieldClaudia E Vickers
Sep 4, 2021·Journal of Agricultural and Food Chemistry·Da-Wei WangZhen Xi

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Datasets Mentioned

BETA
KR013127
AY097402
BAF11220

Methods Mentioned

BETA
size exclusion chromatography
microscale thermophoresis
transgenic
nuclear magnetic resonance
X‐ray
NMR
pulldown
PCR
gel filtration
static light scattering

Software Mentioned

PRIMUS
GraphPad Prism
GraphPad ( Prism
SASPLOT
CRYSOL
ATSAS
Phenix Refine
SWING
Coot
Topspin

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