The high-resolution NMR structure of apolipophorin III from the sphinx moth, Manduca sexta, has been determined in the lipid-free state. We show that lipid-free apolipophorin III adopts a unique helix-bundle topology that has several characteristic structural features. These include a marginally stable, up-and-down helix bundle that allows for concerted opening of the bundle about "hinged" loops upon lipid interaction and buried polar/ionizable residues and buried interhelical H-bonds located in the otherwise hydrophobic interior of the bundle that adjust protein stability and facilitate lipid-induced conformational opening. We suggest that these structural features modulate the conformational adaptability of the lipid-free helix bundle upon lipid binding and control return of the open conformation to the original lipid-free helix-bundle state. Taken together, these data provide a structural rationale for the ability of exchangeable apolipoproteins to reversibly interact with circulating lipoprotein particles.
Improved methods for building protein models in electron density maps and the location of errors in these models
Identification and localization of two distinct microenvironments for the diacylglycerol component of lipophorin particles by 13C NMR
Low concentrations of diacylglycerol promote the binding of apolipophorin III to a phospholipid bilayer: a surface plasmon resonance spectroscopy study
Thermal unfolding of human high-density apolipoprotein A-1: implications for a lipid-free molten globular state
Hydrogen/deuterium exchange kinetics of apolipophorin-III in lipid-free and phospholipid-bound states. An analysis by Fourier transform infrared spectroscopy.
Disulfide bond engineering to monitor conformational opening of apolipophorin III during lipid binding.
Insight into lipid surface recognition and reversible conformational adaptations of an exchangeable apolipoprotein by multidimensional heteronuclear NMR techniques.
Protein backbone angle restraints from searching a database for chemical shift and sequence homology
Conformation of human apolipoprotein C-I in a lipid-mimetic environment determined by CD and NMR spectroscopy
Lipid-triggered conformational switch of apolipophorin III helix bundle to an extended helix organization
The extent of pyrene excimer fluorescence emission is a reflector of distance and flexibility: analysis of the segment linking the LDL receptor-binding and tetramerization domains of apolipoprotein E3
Translocator protein (18 kDa) (TSPO) as a therapeutic target for neurological and psychiatric disorders
The low resolution structure of ApoA1 in spherical high density lipoprotein revealed by small angle neutron scattering.
A unified scheme for initiation and conformational adaptation of human apolipoprotein E N-terminal domain upon lipoprotein binding and for receptor binding activity
Apolipoprotein structural organization in high density lipoproteins: belts, bundles, hinges and hairpins
Lipopolysaccharide binding of an exchangeable apolipoprotein, apolipophorin III, from Galleria mellonella
Crystal structure of human apolipoprotein A-I: insights into its protective effect against cardiovascular diseases
Cholesterol transport in steroid biosynthesis: role of protein-protein interactions and implications in disease states
Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein
Molecular characterization and gene expression of apolipophorin III from the ghost moth, Thitarodes pui (Lepidoptera, Hepialidae)
Molecular characterization of an Apolipophorin-III gene from the Chinese oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)
Apolipophorin III interaction with model membranes composed of phosphatidylcholine and sphingomyelin using differential scanning calorimetry
Contributions of domain structure and lipid interaction to the functionality of exchangeable human apolipoproteins
Comprehensive profiling of lysine acetylation suggests the widespread function is regulated by protein acetylation in the silkworm, Bombyx mori
The lipid composition of Legionella dumoffii membrane modulates the interaction with Galleria mellonella apolipophorin III
Involvement of a versatile pattern recognition receptor, apolipophorin-III in prophenoloxidase activation and antibacterial defense of the Chinese oak silkworm, Antheraea pernyi
An entomopathogenic bacterium, Xenorhabdus nematophila, inhibits hemocyte phagocytosis of Spodoptera exigua by inhibiting phospholipase A(2)
New insights into the determination of HDL structure by apolipoproteins: Thematic review series: high density lipoprotein structure, function, and metabolism.
How the lipid-free structure of the N-terminal truncated human apoA-I converts to the lipid-bound form: new insights from NMR and X-ray structural comparison
Secretome Analysis and In Planta Expression of Salivary Proteins Identify Candidate Effectors from the Brown Planthopper Nilaparvata lugens
A Biomphalaria glabrata peptide that stimulates significant behaviour modifications in aquatic free-living Schistosoma mansoni miracidia
NMR solution structure and dynamics of an exchangeable apolipoprotein, Locusta migratoria apolipophorin III.
Lipid-bound apoLp-III is less effective in binding to lipopolysaccharides and phosphatidylglycerol vesicles compared to the lipid-free protein
Specific sequences in the N and C termini of apolipoprotein A-IV modulate its conformation and lipid association
Structure-function relationships of apolipoprotein A-I: a flexible protein with dynamic lipid associations
Membrane binding of beta2-glycoprotein I can be described by a two-state reaction model: an atomic force microscopy and surface plasmon resonance study
Apolipophorin III interaction with phosphatidylglycerol and lipopolysaccharide: a potential mechanism for antimicrobial activity
ApoE, Lipids & Cholesterol
Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.