Structural basis for the mechanism and substrate specificity of glycocyamine kinase, a phosphagen kinase family member.

Biochemistry
Kap LimOsnat Herzberg

Abstract

Glycocyamine kinase (GK), a member of the phosphagen kinase family, catalyzes the Mg(2+)-dependent reversible phosphoryl group transfer of the N-phosphoryl group of phosphoglycocyamine to ADP to yield glycocyamine and ATP. This reaction helps to maintain the energy homeostasis of the cell in some multicelullar organisms that encounter high and variable energy turnover. GK from the marine worm Namalycastis sp. is heterodimeric, with two homologous polypeptide chains, alpha and beta, derived from a common pre-mRNA by mutually exclusive N-terminal alternative exons. The N-terminal exon of GKbeta encodes a peptide that is different in sequence and is 16 amino acids longer than that encoded by the N-terminal exon of GKalpha. The crystal structures of recombinant GKalphabeta and GKbetabeta from Namalycastis sp. were determined at 2.6 and 2.4 A resolution, respectively. In addition, the structure of the GKbetabeta was determined at 2.3 A resolution in complex with a transition state analogue, Mg(2+)-ADP-NO(3)(-)-glycocyamine. Consistent with the sequence homology, the GK subunits adopt the same overall fold as that of other phosphagen kinases of known structure (the homodimeric creatine kinase (CK) and the monomeric arginine kinase (A...Continue Reading

References

Jul 6, 1990·Biochimica Et Biophysica Acta·X C WangC L Tsou
Jan 1, 1980·Annual Review of Biochemistry·J R Knowles
Jun 1, 1995·The Biochemical Journal·P Kaldis, T Wallimann
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·G ZhouM S Chapman
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Dec 16, 1998·FEBS Letters·J K RaoA Wlodawer
Dec 14, 1999·Protein Science : a Publication of the Protein Society·M EderK Fritz-Wolf
Jul 19, 2000·Biochimica Et Biophysica Acta·T HornemannT Wallimann
Jan 3, 2001·Acta Crystallographica. Section D, Biological Crystallography·M D WinnG N Murshudov
Feb 15, 2001·Acta Crystallographica. Section D, Biological Crystallography·D TisiA Loew
Feb 22, 2001·Annual Review of Physiology·W R Ellington
Aug 24, 2001·Acta Crystallographica. Section D, Biological Crystallography·Y Q ShenZ J Lin
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Nov 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Mohammad S YousefMichael S Chapman
Dec 21, 2002·Protein Science : a Publication of the Protein Society·Mohammad S YousefMichael S Chapman
Feb 24, 2004·Protein Science : a Publication of the Protein Society·Arezki AzziMichael S Chapman
Mar 3, 2004·Acta Crystallographica. Section D, Biological Crystallography·Laurent C StoroniRandy J Read
Oct 16, 2004·Journal of Molecular Evolution·Tomohiko SuzukiW Ross Ellington
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·T Alwyn Jones
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Feb 8, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Chisa MizutaTomohiko Suzuki
Apr 5, 2005·Critical Reviews in Biochemistry and Molecular Biology·Michael J McLeish, George L Kenyon
Apr 5, 2005·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoyRandy J Read
Apr 29, 2005·BMC Bioinformatics·Erik L L Sonnhammer, Volker Hollich
Jul 23, 2005·IUBMB Life·Pan-Fen WangGeorge L Kenyon
Dec 3, 2005·Protein Science : a Publication of the Protein Society·Sreedevi NallamsettyDavid S Waugh
Mar 1, 2007·Acta Crystallographica. Section D, Biological Crystallography·Jeffrey F OhrenRonald E Viola
Jun 19, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Brenda C HeldSteven H Grossman
Oct 13, 2007·Journal of Molecular Evolution·Kumiko TanakaTomohiko Suzuki
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Jan 8, 2011·The Journal of Biological Chemistry·D Jeffrey BushMichael S Chapman
Aug 28, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Allyson PalmerDean Fraga
Sep 22, 2012·Biochemical and Biophysical Research Communications·Shawn A ClarkMichael S Chapman
Aug 10, 2015·Journal of Bioenergetics and Biomembranes·Sergej M Ostojic
Jun 7, 2015·International Journal of Biological Macromolecules·Noriko OkazakiTomohiko Suzuki
Apr 8, 2015·Acta Crystallographica. Section D, Biological Crystallography·Zhiming WangRongguang Zhang
Jun 19, 2016·International Journal of Biological Macromolecules·Xiao-Yu ShiYong-Doo Park
May 13, 2017·Journal of Structural Biology·Omar DavulcuMichael S Chapman
Mar 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Janusz J PetkowskiSara Seager
May 30, 2020·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Paige PiperDean Fraga
Aug 10, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Daichi YanoTomohiko Suzuki

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