Structural basis for the metal-selective activation of the manganese transport regulator of Bacillus subtilis

Biochemistry
Joseph I KliegmanArthur Glasfeld

Abstract

The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium and zinc. The previously published MntR.Mn(2+) structure revealed a binuclear complex of manganese ions with a metal-metal separation of 3.3 A (herein designated the AB conformer). Analysis of four additional crystal forms of MntR.Mn(2+) reveals that the AB conformer is only observed in monoclinic crystals at 100 K, suggesting that this conformation may be stabilized by crystal packing forces. In contrast, monoclinic crystals analyzed at room temperature (at either pH 6.5 or pH 8.5), and a second hexagonal crystal form (analyzed at 100 K), all reveal the shift of one manganese ion by 2.5 A, thereby leading to a newly identified conformation (the AC conformer) with an internuclear distance of 4.4 A. Significantly, the cadmium and calcium complexes of MntR also contain binuclear complexes with a 4.4 A internuclear separation. In contrast, the zinc complex of MntR contains only one metal ion per subunit, in the A site....Continue Reading

References

Feb 1, 1966·Biochemistry·N E GoodR M Singh
Jun 1, 1996·Molecular Endocrinology·J R LundbladR H Goodman
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M C WilceJ A Wilce
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
May 5, 1999·Current Opinion in Chemical Biology·C F Yocum, V L Pecoraro
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·J W Pflugrath
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·G C DismukesA Tyryshkin
Aug 24, 2001·Journal of Molecular Biology·D H Juers, B W Matthews
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·P D TwiggD L Caspar
Dec 25, 2002·The Tohoku Journal of Experimental Medicine·Seiichiro HimenoNobumasa Imura
Feb 6, 2003·Angewandte Chemie·William F DeGradoLucio Randaccio
Mar 13, 2003·Chemical Reviews·Todor Dudev, Carmay Lim
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michelle M SpieringMichael A Marletta
Apr 5, 2003·Molecular Microbiology·Emmanuel Guedon, John D Helmann
Jun 28, 2003·FEMS Microbiology Reviews·David G Kehres, Michael E Maguire
Jul 9, 2003·Nature Structural Biology·Arthur GlasfeldRichard G Brennan
Sep 6, 2003·Science·Anita ChangelaAlfonso Mondragón
Sep 13, 2003·Nature Structural Biology·Eric R SchreiterCatherine L Drennan
Feb 24, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Michelle L Zaharik, B Brett Finlay
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bertil Halle
Apr 24, 2004·Annals of the New York Academy of Sciences·C W Olanow
Oct 8, 2004·Molecular Microbiology·Delfina C Dominguez
Dec 8, 2004·Acta Crystallographica. Section D, Biological Crystallography·Clare E M StevensonDavid M Lawson
Jan 22, 2005·Journal of Bacteriology·Jack S IkedaJames M Slauch
Sep 15, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mario A Pennella, David P Giedroc
Oct 20, 2005·Accounts of Chemical Research·Stephen TotteyNigel J Robinson

❮ Previous
Next ❯

Citations

Dec 21, 2006·Biochemistry·Misha V GolynskiySeth M Cohen
Jan 10, 2013·Biochemistry·Amanda M McGuireArthur Glasfeld
May 29, 2009·Biochemistry·Ellen W MoomawNigel G J Richards
Oct 1, 2009·Chemical Reviews·Zhen MaDavid P Giedroc
Dec 7, 2006·Bioinformatics·Richard ThilakarajGautam Pennathur
Jan 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Samuel A ShelburneJames M Musser
May 6, 2010·Natural Product Reports·Deenah Osman, Jennifer S Cavet
Jul 20, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·David P Giedroc, Alphonse I Arunkumar
Mar 1, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Serenella MediciMaria Antonietta Zoroddu
Mar 8, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Misha GolynskiySeth M Cohen
Dec 19, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Benjamin B BartelleDaniel H Turnbull
Apr 23, 2011·Biophysical Chemistry·Hermes Reyes-CaballeroDavid P Giedroc
Feb 9, 2008·Current Opinion in Chemical Biology·Peng R Chen, Chuan He
Aug 25, 2009·Proteins·Tomoyuki TanakaShigeyuki Yokoyama
Jan 18, 2008·Journal of Molecular Recognition : JMR·Oksana Okhrimenko, Ilian Jelesarov
May 20, 2008·Molecular Microbiology·Samuel A ShelburneJames M Musser
Oct 13, 2012·Molecular Microbiology·Zhen MaJohn D Helmann
Nov 23, 2006·Journal of Molecular Biology·Mark A DeWittArthur Glasfeld
Apr 23, 2015·Molecular Microbiology·Lillian J Juttukonda, Eric P Skaar
Jul 28, 2012·Critical Reviews in Microbiology·Shawn FrenchSusan Glasauer
Nov 3, 2007·Journal of Molecular Biology·Nilakshee BhattacharyaTimothy M Logan
Dec 4, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Maurizio RemelliMaria Antonietta Zoroddu
May 13, 2014·Acta Crystallographica. Section D, Biological Crystallography·Hyun Ku YeoJae Young Lee
Mar 28, 2017·Nature Reviews. Microbiology·Pete ChandrangsuJohn D Helmann
Aug 28, 2014·The Journal of Biological Chemistry·John D Helmann
Jun 13, 2006·The Journal of Biological Chemistry·Jin-Won Lee, John D Helmann
Nov 7, 2019·Journal of Bacteriology·Srinand ParuthiyilJohn D Helmann
May 10, 2020·Trends in Microbiology·Erin E Price, Jeffrey M Boyd
May 29, 2018·Cell Chemical Biology·Sharrol T Bachas, Adrian R Ferré-D'Amaré

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.