Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase.

The Journal of Biological Chemistry
Franck CosteBertrand Castaing

Abstract

Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme that excises oxidized purines such as 7,8-dihydro-8-oxoguanine (8-oxoG) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) from damaged DNA. Here, we report the crystal structure of the Fpg protein from Lactococcus lactis (LlFpg) bound to a carbocyclic FapydG (cFapydG)-containing DNA. The structure reveals that Fpg stabilizes the cFapydG nucleoside into an extrahelical conformation inside its substrate binding pocket. In contrast to the recognition of the 8-oxodG lesion, which is bound with the glycosidic bond in a syn conformation, the cFapydG lesion displays in the complex an anti conformation. Furthermore, Fpg establishes interactions with all the functional groups of the FapyG base lesion, which can be classified in two categories: (i) those specifying a purine-derived lesion (here a guanine) involved in the Watson-Crick face recognition of the lesion and probably contributing to an optimal orientation of the pyrimidine ring moiety in the binding pocket and (ii) those specifying the imidazole ring-opened moiety of FapyG and probably participating also in the rotameric selection of the FapydG nucleobase. These interactions involve strictly conserved Fpg re...Continue Reading

References

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J TchouS Nishimura
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T R O'Connor, J Laval
Aug 1, 1987·British Journal of Cancer·T Lindahl
Aug 5, 1984·Nature·C LagravèreJ Laval
Jun 1, 1995·Free Radical Biology & Medicine·A P Breen, J A Murphy
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·L A LipscombL D Williams
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·J P RadicellaS Boiteux
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Dec 24, 1998·Nucleic Acids Research·B CastaingC Zelwer
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Sep 29, 2000·The Journal of Biological Chemistry·J L RavanatJ Cadet
Feb 27, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·O D Schärer, J Jiricny
Feb 2, 2002·Chemistry : a European Journal·Lars T Burgdorf, Thomas Carell
Mar 26, 2002·The Journal of Biological Chemistry·Rotem GilboaGil Shoham
Mar 27, 2002·Acta Crystallographica. Section D, Biological Crystallography·Karine Pereira de JésusBertrand Castaing
Sep 11, 2002·Biochemistry·Yu Lin Jiang, James T Stivers

❮ Previous
Next ❯

Citations

Jun 9, 2005·Mutation Research·Dmitry O Zharkov, Arthur P Grollman
Jun 9, 2005·Mutation Research·Joy L HuffmanJohn A Tainer
Dec 25, 2007·Biochemistry·Nirmala KrishnamurthySheila S David
Aug 18, 2012·Chemical Research in Toxicology·Mark LukinCarlos de Los Santos
Jan 24, 2013·Chemical Research in Toxicology·Gergely Morten SoleckiMelanie Esselen
Nov 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jongchan YeoPeter A Beal
Jan 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yan QiGregory L Verdine
Jul 25, 2009·The Journal of Biological Chemistry·Kayo ImamuraSylvie Doublié
Apr 3, 2012·The Journal of Biological Chemistry·Rou-Jia SungGregory L Verdine
Apr 14, 2011·Nucleic Acids Research·Yann-Vaï Le BihanBertrand Castaing
Sep 11, 2008·Journal of the Royal Society, Interface·Supat Jiranusornkul, Charles A Laughton
Oct 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anirban Banerjee, Gregory L Verdine
Jun 22, 2010·Analytical and Bioanalytical Chemistry·Elijah J Petersen, Bryant C Nelson
Jan 1, 2013·Mutation Research·Minmin LiuSusan S Wallace
Aug 4, 2012·DNA Repair·Erik Sebastian VikBjørn Dalhus
May 16, 2012·Free Radical Biology & Medicine·N R Jena, P C Mishra
Aug 12, 2008·Free Radical Biology & Medicine·Miral DizdarogluPawel Jaruga
Mar 27, 2007·Molecular Aspects of Medicine·Guido Frosina
Oct 15, 2013·Environmental and Molecular Mutagenesis·Susan S Wallace
Oct 19, 2012·Biochimica Et Biophysica Acta·Sonja C BrooksBrandt F Eichman
Jan 27, 2012·Free Radical Research·Miral Dizdaroglu, Pawel Jaruga
Aug 8, 2009·FEMS Microbiology Reviews·Bjørn DalhusMagnar Bjørås
Feb 25, 2010·Current Biology : CB·Josef Jiricny
Feb 10, 2009·Current Opinion in Structural Biology·Sabine SchneiderThomas Carell
May 28, 2015·Scientific Reports·Iris D BlankChristian Ochsenfeld
May 19, 2017·The Journal of Physical Chemistry. B·Namrata Jayanth, Mrinalini Puranik

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.