PMID: 8605630Mar 1, 1996Paper

Structural basis for the root effect in haemoglobin

Nature Structural Biology
S E MylvaganamE D Getzoff

Abstract

The remarkable ability of root effect haemoglobins to pump oxygen against high O2 gradients results from extreme, acid-induced reductions in O2 affinity and cooperativity. The long-sought mechanism for the root effect, revealed by the 2 angstrom crystal structure of the ligand-bound haemoglobin from Leiostomus xanthurus at pH 7.5, unexpectedly involves modulation of the R-state. Key residues strategically assemble positive-charge clusters across the allosteric beta1 beta2-interface in the R-state. At low pH, protonation of the beta N terminus and His 147(HC3)beta within these clusters is postulated to destabilize the R-state and promote the acid-triggered, allosteric R-->T switch with concomitant O2 release. Surprisingly, a set of residues specific to root effect haemoglobins recruit additional residues, conserved among most haemoglobins, to produce the root effect.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M BrunoriJ Wyman
Apr 5, 1979·Journal of Molecular Biology·J Baldwin, C Chothia
Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P A Mourão, C P Dietrich
Aug 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G di Prisco, M Tamburrini
Aug 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C CarusoG di Prisco
Jun 24, 1991·Biochimica Et Biophysica Acta·C CarusoG di Prisco
Oct 1, 1990·Journal of Molecular Evolution·K HorimotoJ Otsuka
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T Brittain
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K NagaiC Poyart
Nov 17, 1983·Nature·J A TainerD C Richardson
Nov 25, 1983·Journal of Molecular Biology·B Shaanan
Jan 15, 1980·Journal of Molecular Biology·J M Baldwin
Jun 17, 1994·Journal of Molecular Biology·M F PerutzD Williamson
Apr 20, 1993·Journal of Molecular Biology·D T ShihK Nagai
Jan 23, 1987·Science·A T BrüngerM Karplus

❮ Previous
Next ❯

Citations

Mar 10, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Matthew D Regan, Colin J Brauner
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Bernd Pelster, Heinz Decker
Sep 29, 1999·Trends in Biochemical Sciences·J R Tame
Mar 20, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·D J Randall, C Brauner
Feb 6, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mônica B MeloFernando F Costa
Mar 1, 1996·Nature Structural Biology·M F Perutz
Apr 1, 1997·Nature Structural Biology·K Diederichs, P A Karplus
Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kenichi HitomiElizabeth D Getzoff
Jul 9, 2010·The Journal of Biological Chemistry·Alessandro VergaraLelio Mazzarella
Nov 4, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Antonello MerlinoAlessandro Vergara
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·D WheelerR A Holland
Sep 1, 2009·Marine Genomics·Katrín Halldórsdóttir, Einar Arnason
Oct 20, 2004·Acta Physiologica Scandinavica·C BonaventuraR E Weber
Oct 30, 2013·The FEBS Journal·Maurizio Brunori
Nov 24, 2004·Respiratory Physiology & Neurobiology·Roy E Weber, Angela Fago
Feb 5, 2013·Biochimica Et Biophysica Acta·Luca RondaAlessandro Vergara
May 14, 2011·Biochimica Et Biophysica Acta·Andrea Bellelli, Maurizio Brunori
Aug 2, 2001·The Journal of Experimental Zoology·T H Waterman
Jun 22, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P C de Souza, G O Bonilla-Rodriguez
May 1, 2004·The Journal of Biological Chemistry·Takeshi YokoyamaSam-Yong Park
Jul 22, 2018·The Journal of Experimental Biology·Agnieszka JendroszekAngela Fago
Sep 14, 2000·The Journal of Biological Chemistry·C H TaboyA L Crumbliss
Jul 25, 1998·The Journal of Biological Chemistry·J M ManningL R Manning
Jan 11, 2019·Fish Physiology and Biochemistry·Manuela ManiaSalvatore Campo
Jul 17, 2001·European Journal of Biochemistry·M TamburriniM Pellegrini
Sep 17, 2005·The Journal of Biological Chemistry·Celia BonaventuraRobert Henkens
Nov 24, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·B Pelster
Apr 15, 2020·Analytical Biochemistry·Lois R Manning, James M Manning
May 1, 1999·Journal of Molecular Biology·L MazzarellaA Zagari
Aug 19, 2004·Journal of Agricultural and Food Chemistry·Hordur G Kristinsson, Herbert O Hultin
May 27, 2004·Journal of Agricultural and Food Chemistry·Hordur G Kristinsson, Herbert O Hultin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.