Structural basis of σ appropriation

Nucleic Acids Research
Jing ShiYu Feng

Abstract

Bacteriophage T4 middle promoters are activated through a process called σ appropriation, which requires the concerted effort of two T4-encoded transcription factors: AsiA and MotA. Despite extensive biochemical and genetic analyses, puzzle remains, in part, because of a lack of precise structural information for σ appropriation complex. Here, we report a single-particle cryo-electron microscopy (cryo-EM) structure of an intact σ appropriation complex, comprising AsiA, MotA, Escherichia coli RNA polymerase (RNAP), σ70 and a T4 middle promoter. As expected, AsiA binds to and remodels σ region 4 to prevent its contact with host promoters. Unexpectedly, AsiA undergoes a large conformational change, takes over the job of σ region 4 and provides an anchor point for the upstream double-stranded DNA. Because σ region 4 is conserved among bacteria, other transcription factors may use the same strategy to alter the landscape of transcription immediately. Together, the structure provides a foundation for understanding σ appropriation and transcription activation.

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Citations

Jan 16, 2020·Biomolecular NMR Assignments·Zhihao WangBing Liu
Aug 28, 2020·Frontiers in Microbiology·Rishi Kishore Vishwakarma, Konstantin Brodolin
Oct 31, 2019·Transcription·Markus C Wahl, Ranjan Sen
Jan 1, 2021·The Journal of Biological Chemistry·Konstantin Brodolin, Zakia Morichaud
Oct 31, 2020·Nature Reviews. Microbiology·James ChenElizabeth A Campbell
Jan 12, 2021·The Journal of Biological Chemistry·Konstantin Brodolin, Zakia Morichaud
Sep 17, 2021·Nucleic Acids Research·Jing ShiWei Lin

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Datasets Mentioned

BETA
EMD-9916

Methods Mentioned

BETA
NMR
gel filtration
fluorescence polarization assay
surface plasmon resonance
in vitro transcription

Software Mentioned

WebLogo
ImageJ
Chimera
RELION
CTFFIND4
Phenix

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